Document Detail


The human milk oligosaccharide disialyllacto-N-tetraose prevents necrotising enterocolitis in neonatal rats.
MedLine Citation:
PMID:  22138535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Necrotising enterocolitis (NEC) is one of the most common and fatal intestinal disorders in preterm infants. Breast-fed infants are at lower risk for NEC than formula-fed infants, but the protective components in human milk have not been identified. In contrast to formula, human milk contains high amounts of complex glycans.
OBJECTIVE: To test the hypothesis that human milk oligosaccharides (HMO) contribute to the protection from NEC.
METHODS: Since human intervention studies are unfeasible due to limited availability of HMO, a neonatal rat NEC model was used. Pups were orally gavaged with formula without and with HMO and exposed to hypoxia episodes. Ileum sections were scored blindly for signs of NEC. Two-dimensional chromatography was used to determine the most effective HMO, and sequential exoglycosidase digestions and linkage analysis was used to determine its structure.
RESULTS: Compared to formula alone, pooled HMO significantly improved 96-hour survival from 73.1% to 95.0% and reduced pathology scores from 1.98 ± 1.11 to 0.44 ± 0.30 (p<0.001). Within the pooled HMO, a specific isomer of disialyllacto-N-tetraose (DSLNT) was identified to be protective. Galacto-oligosaccharides, currently added to formula to mimic some of the effects of HMO, had no effect.
CONCLUSION: HMO reduce NEC in neonatal rats and the effects are highly structure specific. If these results translate to NEC in humans, DSLNT could be used to prevent or treat NEC in formula-fed infants, and its concentration in the mother's milk could serve as a biomarker to identify breast-fed infants at risk of developing this disorder.
Authors:
Evelyn Jantscher-Krenn; Monica Zherebtsov; Caroline Nissan; Kerstin Goth; Yigit S Guner; Natasha Naidu; Biswa Choudhury; Anatoly V Grishin; Henri R Ford; Lars Bode
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-12-03
Journal Detail:
Title:  Gut     Volume:  61     ISSN:  1468-3288     ISO Abbreviation:  Gut     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-10     Completed Date:  2012-11-19     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  2985108R     Medline TA:  Gut     Country:  England    
Other Details:
Languages:  eng     Pagination:  1417-25     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Disease Models, Animal
Enterocolitis, Necrotizing / mortality,  pathology,  prevention & control*
Gas Chromatography-Mass Spectrometry
Humans
Milk, Human / chemistry*
Oligosaccharides / analysis,  therapeutic use*
Random Allocation
Rats
Rats, Sprague-Dawley
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Grant Support
ID/Acronym/Agency:
K99/R00 DK078668/DK/NIDDK NIH HHS; R01 AI014032/AI/NIAID NIH HHS; R01 AI014032/AI/NIAID NIH HHS; R03 HD059717S1/HD/NICHD NIH HHS; R21 AI083612/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Oligosaccharides; 0/disialyllacto-N-tetraose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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