Document Detail


The human homolog of the Drosophila headcase protein slows down cell division of head and neck cancer cells.
MedLine Citation:
PMID:  19643820     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human homolog of the Drosophila headcase (HECA) belongs to a new class of cell differentiation regulators. In Drosophila, the HECA protein regulates the proliferation and differentiation of cells during adult morphogenesis. There is growing evidence that HECA plays an important role in human carcinogenesis. In different tumor entities, an altered HECA expression was found (colorectal, pancreatic and renal cancer). Colorectal cancer studies also suggested HECA as a marker for early disease stages. Therefore, we speculated whether human HECA affects cell cycle progression and proliferation in head and neck cancer cells. In vivo, we found a distinct HECA protein expression in basal and superficial cells of a healthy oral epithelium via immunohistochemistry, whereas in tissues of oral squamous cell carcinoma (OSCC), a weaker staining was observed, particularly in basal cells. In vitro, mRNA and protein expression analyses of OSCC cell lines exhibited that HECA expression correlates with the state of cellular differentiation. In further investigations, we overexpressed HECA in the OSCC cell line PCI 13 and performed functional assays. HECA-overexpressing OSCC cells revealed a significant extended doubling time (up to 45%, 17 h) and yielded a lower number of proliferating cells (up to 30%) than controls. Flow cytometry analyses have shown that HECA-overexpressing OSCC cells forced to hold in the G(2)/M-Phase. In summary, our results show that human HECA slows down cell division of OSCC cells and may therefore act as a tumor suppressor in head and neck cancer.
Authors:
Albert Dowejko; Richard J Bauer; Urs D A Müller-Richter; Torsten E Reichert
Publication Detail:
Type:  Journal Article     Date:  2009-07-30
Journal Detail:
Title:  Carcinogenesis     Volume:  30     ISSN:  1460-2180     ISO Abbreviation:  Carcinogenesis     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-06     Completed Date:  2009-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  England    
Other Details:
Languages:  eng     Pagination:  1678-85     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, University of Regensburg, Regensburg, Germany. albert.dowejko@klinik.uni-regensburg.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Apoptosis
Carcinoma, Squamous Cell / genetics*,  pathology
Cell Cycle
Cell Division
Cell Line
Cell Line, Tumor
DNA Primers
Drosophila / genetics
Drosophila Proteins / genetics
Flow Cytometry
Head and Neck Neoplasms / genetics,  pathology*
Humans
Keratinocytes / cytology,  physiology
Kinetics
Morphogenesis / genetics
Mouth Neoplasms / genetics,  pathology
Neoplasm Proteins / genetics*
Neoplasm Staging
Plasmids
Polymerase Chain Reaction
RNA, Messenger / genetics
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Drosophila Proteins; 0/HECA protein, human; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/headcase protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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