| The human homolog of the Drosophila headcase protein slows down cell division of head and neck cancer cells. | |
| | |
MedLine Citation:
|
PMID: 19643820 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The human homolog of the Drosophila headcase (HECA) belongs to a new class of cell differentiation regulators. In Drosophila, the HECA protein regulates the proliferation and differentiation of cells during adult morphogenesis. There is growing evidence that HECA plays an important role in human carcinogenesis. In different tumor entities, an altered HECA expression was found (colorectal, pancreatic and renal cancer). Colorectal cancer studies also suggested HECA as a marker for early disease stages. Therefore, we speculated whether human HECA affects cell cycle progression and proliferation in head and neck cancer cells. In vivo, we found a distinct HECA protein expression in basal and superficial cells of a healthy oral epithelium via immunohistochemistry, whereas in tissues of oral squamous cell carcinoma (OSCC), a weaker staining was observed, particularly in basal cells. In vitro, mRNA and protein expression analyses of OSCC cell lines exhibited that HECA expression correlates with the state of cellular differentiation. In further investigations, we overexpressed HECA in the OSCC cell line PCI 13 and performed functional assays. HECA-overexpressing OSCC cells revealed a significant extended doubling time (up to 45%, 17 h) and yielded a lower number of proliferating cells (up to 30%) than controls. Flow cytometry analyses have shown that HECA-overexpressing OSCC cells forced to hold in the G(2)/M-Phase. In summary, our results show that human HECA slows down cell division of OSCC cells and may therefore act as a tumor suppressor in head and neck cancer. |
| | |
Authors:
|
Albert Dowejko; Richard J Bauer; Urs D A Müller-Richter; Torsten E Reichert |
Publication Detail:
|
Type: Journal Article Date: 2009-07-30 |
Journal Detail:
|
Title: Carcinogenesis Volume: 30 ISSN: 1460-2180 ISO Abbreviation: Carcinogenesis Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-10-06 Completed Date: 2009-10-26 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: England |
Other Details:
|
Languages: eng Pagination: 1678-85 Citation Subset: IM |
Affiliation:
|
Department of Oral and Maxillofacial Surgery, University of Regensburg, Regensburg, Germany. albert.dowejko@klinik.uni-regensburg.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Animals Apoptosis Carcinoma, Squamous Cell / genetics*, pathology Cell Cycle Cell Division Cell Line Cell Line, Tumor DNA Primers Drosophila / genetics Drosophila Proteins / genetics Flow Cytometry Head and Neck Neoplasms / genetics, pathology* Humans Keratinocytes / cytology, physiology Kinetics Morphogenesis / genetics Mouth Neoplasms / genetics, pathology Neoplasm Proteins / genetics* Neoplasm Staging Plasmids Polymerase Chain Reaction RNA, Messenger / genetics |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/Drosophila Proteins; 0/HECA protein, human; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/headcase protein, Drosophila |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interaction between cytochrome P450 1A2 genetic polymorphism and cigarette smoking on the risk of he...
Next Document: Urinary bladder carcinogenesis induced by chronic exposure to persistent low-dose ionising radiation...