| A human RNA polymerase II-containing complex associated with factors necessary for spliceosome assembly. | |
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MedLine Citation:
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PMID: 11773074 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transcription and splicing are coordinated processes in mammalian cells. We have used affinity chromatography with immobilized transcription elongation factor SII to purify a protein complex that contains core RNA polymerase II (RNA Pol II), the general transcription initiation factors, and several splicing factors, including the U1, U2, and U4 small nuclear RNPs, the U2AF(65), and serine/arginine-rich proteins. The splicing factors and the transcription machinery co-purify through a gel filtration column and co-immunoprecipitate in experiments using an anti-U2AF(65) antibody, indicating that they are part of a unique complex. Although the RNA Pol II-containing complex does not possess splicing activity, it can complement small nuclear RNP-inactivated extracts and can promote the formation of a pre-spliceosome complex. Because interactions between components of the splicing and transcription machineries occur in the context of a complex containing a hypophosphorylated RNA Pol II capable of initiating transcription, our results suggest that the coupling between transcription and splicing begins before transcription initiation. |
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Authors:
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Francois Robert; Marco Blanchette; Olivier Maes; Benoit Chabot; Benoit Coulombe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2001-12-31 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 277 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-03-11 Completed Date: 2002-04-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 9302-6 Citation Subset: IM |
Affiliation:
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Laboratory of Gene Transcription, Institut de Recherches Cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, Québec H2W 1R7, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Affinity Humans Phosphorylation RNA Polymerase II / chemistry*, isolation & purification RNA Splicing* Spliceosomes / chemistry* Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.-/RNA Polymerase II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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