| The human Pim-2 proto-oncogene and its testicular expression. | |
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MedLine Citation:
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PMID: 9804974 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we describe the cloning of a human gene, encoding a protein that shares 90% identity and 93% similarity at the primary structure level, with the mouse Pim-2 gene. The gene was designated hPim-2. Structural features suggest that like the mouse Pim-2, hPim-2 is also a serine threonine kinase. At the RNA level, two hPim-2 transcripts were identified. The first, 2.2 kb, is highly expressed in hematopoietic tissues and in leukemic and lymphoma cell lines (K-562, HL-60 and RAJI). It also shows considerable high levels in testis, small intestine, colon and human colorectal adenocarcinoma cells (SW480). A second transcript, 5.0 kb in size, could be detected only in spleen, thymus, small intestine and colon and in the K-562 and RAJI cell lines. In situ hybridization analysis of biopsies taken from testes of men with complete or partial spermatogenesis revealed that the gene is expressed in primary spermatocytes. In the absence of germ cells, signal could be detected over specific cells in the well developed interstitial region. These results suggest a role for hPim-2 in proliferating cells as well as during meiosis. A possible connection between hPim-2 and apoptosis is discussed. |
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Authors:
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D Baytel; S Shalom; I Madgar; R Weissenberg; J Don |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1442 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1998-12-17 Completed Date: 1998-12-17 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 274-85 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U77735 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma Amino Acid Sequence Animals Base Sequence Colonic Neoplasms Colorectal Neoplasms HL-60 Cells Hematopoietic Stem Cells / enzymology Humans In Situ Hybridization Intestinal Neoplasms K562 Cells Male Mice Molecular Sequence Data Protein-Serine-Threonine Kinases / genetics* Proto-Oncogene Proteins / biosynthesis, chemistry, genetics* Proto-Oncogenes* Recombinant Proteins / biosynthesis, chemistry Sequence Alignment Sequence Homology, Amino Acid Testicular Neoplasms Testis / metabolism* Transcription, Genetic* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/PIM2 protein, human; 0/Pim2 protein, mouse; 0/Proto-Oncogene Proteins; 0/Recombinant Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
| Comments/Corrections | |
Erratum In:
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Biochim Biophys Acta 1999 Feb 16;1444(2):312-3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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