| hth maintains the pool of eye progenitors and its downregulation by Dpp and Hh couples retinal fate acquisition with cell cycle exit. | |
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MedLine Citation:
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PMID: 20036228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During Drosophila eye development, recruitment of retinal precursors from a pool of progenitor cells is tightly coupled to proliferation control. However, how this coupling operates is still unclear. Here we show that the transcription factor hth, together with eyeless, is required to stimulate proliferation of progenitor cells. Accordingly, knocking down hth expression results in severely reduced eyes. Our experiments reveal three additional functions for hth: the cell cycle of progenitors is characterized by a relatively long G2 phase, which makes them prone to enter mitosis; hth represses the burst of string/cdc25 expression that precedes G1 arrest, and also the early expression of the proneural gene atonal. Thereby, hth maintains the proliferative and undifferentiated state of eye progenitors. Furthermore, we show that the G1 synchronization that characterizes retinal precursors is the result of the spatially controlled repression of hth by Dpp and Hh, and not of an actively induced cell cycle arrest. We integrate these results in a model of the early steps of eye development that links proliferation control and differential gene expression with patterning signals. |
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Authors:
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Carla S Lopes; Fernando Casares |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-28 |
Journal Detail:
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Title: Developmental biology Volume: 339 ISSN: 1095-564X ISO Abbreviation: Dev. Biol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: United States |
Other Details:
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Languages: eng Pagination: 78-88 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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CABD-Centro Andaluz de Biologia del Desarrollo Univ. Pablo de Olavide-CSIC, Ctra. Utrera Km 1, Sevilla 41013, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle* Cell Lineage Down-Regulation* Drosophila Drosophila Proteins / genetics, physiology* Hedgehog Proteins / physiology* Homeodomain Proteins / genetics, physiology* Retina / cytology, embryology* Stem Cells / cytology |
| Chemical | |
Reg. No./Substance:
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0/Drosophila Proteins; 0/Hedgehog Proteins; 0/Homeodomain Proteins; 0/dpp protein, Drosophila; 0/homothorax protein, Drosophila; 149291-21-4/hedgehog protein, Drosophila |
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