Document Detail


A homologous naturally occurring mutation in Duffy and CCR5 leading to reduced receptor expression.
MedLine Citation:
PMID:  11369664     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genetic variations in the CC chemokine receptor (CCR5) leading to reduced or absent expression are associated with resistance to human immunodeficiency virus infection and delayed onset of acquired immunodeficiency syndrome. Similarly, lack of the red-cell chemokine receptor Duffy confers protection against malarial infection by Plasmodium vivax. Investigators have previously described a missense mutation (R89C) in the first intracellular loop of Duffy that results in reduced protein expression. The present study shows that the lower Duffy expression is due to loss of the positive charge at this position, resulting in protein instability. Moreover, R60S, a mutation in the first intracellular loop of CCR5 noted in a recent cohort study, likewise results in reduced surface expression and function of CCR5. The presence of a homologous, naturally occurring mutation that may be protective against disease thus defines a novel mechanism accounting for the decreased expression of these receptors in some individuals. (Blood. 2001;97:3651-3654)
Authors:
D Tamasauskas; V Powell; K Saksela; K Yazdanbakhsh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  97     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-05-22     Completed Date:  2001-06-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3651-4     Citation Subset:  AIM; IM    
Affiliation:
Immunochemistry Laboratory, New York Blood Center, 310 East 67th St., New York, NY 10021, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Protozoan*
Calcium / metabolism
Carrier Proteins / genetics*,  physiology
Cell Line
Duffy Blood-Group System / genetics*,  physiology
Gene Expression*
HIV-1 / physiology
Humans
Immunoblotting
Malaria / prevention & control
Mutagenesis, Site-Directed
Mutation*
Protozoan Proteins*
Receptors, CCR5 / genetics*,  physiology
Receptors, Cell Surface / genetics*,  physiology
Transfection
Grant Support
ID/Acronym/Agency:
HL54459/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Protozoan; 0/Carrier Proteins; 0/Duffy Blood-Group System; 0/Duffy antigen binding protein, Plasmodium; 0/Protozoan Proteins; 0/Receptors, CCR5; 0/Receptors, Cell Surface; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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