Document Detail

The homologous chromosome is an effective template for the repair of mitotic DNA double-strand breaks in Drosophila.
MedLine Citation:
PMID:  14704169     Owner:  NLM     Status:  MEDLINE    
In recombinational DNA double-strand break repair a homologous template for gene conversion may be located at several different genomic positions: on the homologous chromosome in diploid organisms, on the sister chromatid after DNA replication, or at an ectopic position. The use of the homologous chromosome in mitotic gene conversion is thought to be limited in the yeast Saccharomyces cerevisiae and mammalian cells. In contrast, by studying the repair of double-strand breaks generated by the I-SceI rare-cutting endonuclease, we find that the homologous chromosome is frequently used in Drosophila melanogaster, which we suggest is attributable to somatic pairing of homologous chromosomes in mitotic cells of Drosophila. We also find that Drosophila mitotic cells of the germ line, like yeast, employ the homologous recombinational repair pathway more often than imperfect nonhomologous end joining.
Yikang S Rong; Kent G Golic
Related Documents :
2680759 - Nonrecombinant meiosis i nondisjunction in saccharomyces cerevisiae induced by trna och...
1867189 - Xy chromosome nondisjunction in man is associated with diminished recombination in the ...
1677769 - Loss of heterozygosity and mitotic linkage maps in the mouse.
12750319 - Positive and negative roles of homologous recombination in the maintenance of genome st...
2843459 - Mapping of igk-v genes using backcrossed laboratory and wild mice.
16175509 - The beta -globin recombinational hotspot reduces the effects of strong selection around...
19901069 - Double-strand break repair pathways protect against cag/ctg repeat expansions, contract...
6885089 - Genetics of plantar interdigital ridge counts.
24221269 - Identification of alien chromatin specifying resistance to wheat streak mosaic and gree...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  165     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-05     Completed Date:  2004-08-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1831-42     Citation Subset:  IM    
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Animals, Genetically Modified
Chromosomes / genetics*
Crosses, Genetic
DNA / metabolism*
DNA Damage
DNA Repair*
Deoxyribonucleases, Type II Site-Specific / metabolism
Drosophila melanogaster / genetics*
Recombination, Genetic*
Saccharomyces cerevisiae Proteins
Signal Transduction
Templates, Genetic
Grant Support
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 9007-49-2/DNA; EC 3.1.21.-/SCEI protein, S cerevisiae; EC, Type II Site-Specific

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Characterization of a male-predominant antisense transcript underexpressed in hybrids of Drosophila ...
Next Document:  Selective constraints on intron evolution in Drosophila.