| The homologous chromosome is an effective template for the repair of mitotic DNA double-strand breaks in Drosophila. | |
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MedLine Citation:
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PMID: 14704169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In recombinational DNA double-strand break repair a homologous template for gene conversion may be located at several different genomic positions: on the homologous chromosome in diploid organisms, on the sister chromatid after DNA replication, or at an ectopic position. The use of the homologous chromosome in mitotic gene conversion is thought to be limited in the yeast Saccharomyces cerevisiae and mammalian cells. In contrast, by studying the repair of double-strand breaks generated by the I-SceI rare-cutting endonuclease, we find that the homologous chromosome is frequently used in Drosophila melanogaster, which we suggest is attributable to somatic pairing of homologous chromosomes in mitotic cells of Drosophila. We also find that Drosophila mitotic cells of the germ line, like yeast, employ the homologous recombinational repair pathway more often than imperfect nonhomologous end joining. |
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Authors:
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Yikang S Rong; Kent G Golic |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Genetics Volume: 165 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2004-01-05 Completed Date: 2004-08-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
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Languages: eng Pagination: 1831-42 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA. rongy@mail.nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Chromosomes / genetics* Crosses, Genetic DNA / metabolism* DNA Damage DNA Repair* Deoxyribonucleases, Type II Site-Specific / metabolism Drosophila melanogaster / genetics* Female Male Mitosis* Recombination, Genetic* Saccharomyces cerevisiae Proteins Signal Transduction Templates, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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GM-5604/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Saccharomyces cerevisiae Proteins; 9007-49-2/DNA; EC 3.1.21.-/SCEI protein, S cerevisiae; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific |
| Comments/Corrections | |
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