Document Detail


The homologous chromosome is an effective template for the repair of mitotic DNA double-strand breaks in Drosophila.
MedLine Citation:
PMID:  14704169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recombinational DNA double-strand break repair a homologous template for gene conversion may be located at several different genomic positions: on the homologous chromosome in diploid organisms, on the sister chromatid after DNA replication, or at an ectopic position. The use of the homologous chromosome in mitotic gene conversion is thought to be limited in the yeast Saccharomyces cerevisiae and mammalian cells. In contrast, by studying the repair of double-strand breaks generated by the I-SceI rare-cutting endonuclease, we find that the homologous chromosome is frequently used in Drosophila melanogaster, which we suggest is attributable to somatic pairing of homologous chromosomes in mitotic cells of Drosophila. We also find that Drosophila mitotic cells of the germ line, like yeast, employ the homologous recombinational repair pathway more often than imperfect nonhomologous end joining.
Authors:
Yikang S Rong; Kent G Golic
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  165     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2004-01-05     Completed Date:  2004-08-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1831-42     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Utah, Salt Lake City, Utah 84112, USA. rongy@mail.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Chromosomes / genetics*
Crosses, Genetic
DNA / metabolism*
DNA Damage
DNA Repair*
Deoxyribonucleases, Type II Site-Specific / metabolism
Drosophila melanogaster / genetics*
Female
Male
Mitosis*
Recombination, Genetic*
Saccharomyces cerevisiae Proteins
Signal Transduction
Templates, Genetic
Grant Support
ID/Acronym/Agency:
GM-5604/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 9007-49-2/DNA; EC 3.1.21.-/SCEI protein, S cerevisiae; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific
Comments/Corrections

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