Document Detail


The homogeneity testing of EtG in hair reference materials: A high-throughput procedure using GC-NCI-MS.
MedLine Citation:
PMID:  23415165     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The validation of a robust quantification procedure for EtG in hair using GC-NCI-MS is presented. Aqueous extraction is followed by complete lyophylization of the extract and derivatization with pentafluoropropionic anhydride (PFPA) under controlled temperature and duration. Clean-up of extracts was dispensable and standard single quadrupole MS displayed sufficient selectivity and sensitivity. The method displayed a wide linearity range and enabled LOD of 0.68pg/mg, LOQ of 2.4pg/mg, and precision below 8.12%. Since EtG was seen to display prolonged stability in the aqueous extracts and after derivatization with PFPA this straightforward procedure allows a routine throughput of large quantities of samples with little proneness to procedural scatter of results. The method was applied to demonstrate the homogeneity of two hair reference materials with mean EtG contents of 8.48pg/mg and 22.0pg/mg. Aside from the application in homogeneity studies of hair reference materials predominantly in the concentration range of 10-50pg/mg the method was also designed for daily routine quantification of real-world sample with regard to drinking behavior assessment.
Authors:
Bettina Mönch; Roland Becker; Christian Jung; Irene Nehls
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-12
Journal Detail:
Title:  Forensic science international     Volume:  -     ISSN:  1872-6283     ISO Abbreviation:  Forensic Sci. Int.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902034     Medline TA:  Forensic Sci Int     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Division of Trace Analysis, Department of Analytical Chemistry, Federal Institute for Materials Research and Testing (BAM), Richard-Willstätter-Strasse 11, 12489 Berlin, Germany.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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