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A homogalacturonan from the radix of Platycodon grandiflorum and the anti-angiogenesis activity of poly-/oligogalacturonic acids derived therefrom.
MedLine Citation:
PMID:  21700274     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A polysaccharide, PGA4-3b, with an average molecular weight of 8.9kDa estimated by high-performance gel-permeation chromatography (HPGPC), was isolated from radix of Platycodon grandiflorum (Jacq.) A. DC. Using monosaccharide analysis, methylation analysis and NMR spectroscopy, PGA4-3b was elucidated to be a linear poly-(1→4)-α-d-galactopyranosyluronic acid that contains no methyl ester groups. Partial acid hydrolysis of PGA4-3b yielded a series of poly- or oligogalacturonic acids with different degrees of polymerization (DP), that is, 4-3bde, 4-3bde-O-1, 4-3bde-O-2, 4-3bde-O-3, and 4-3bde-O-4. Cell tube formation inhibition tests with human microvascular endothelial cells (HMEC) for antiangiogenesis analysis showed that 4-3bde-O-1 and 4-3bde-O-2, the fractions with higher molecular weights, could inhibit tube formation, while the native PGA4-3b and low molecular weight fraction 4-3bde-O-3 and 4-3bde-O4 are ineffective. Moreover, 4-3bde-O-2 with DP 5-10 impaired cell tube formation in a dose-dependent way, suggesting its potential to be developed as an anti-angiogenesis drug. This is the first time oligogalacturonic acids are reported to show an anti-angiogenesis effect.
Authors:
Yuxia Xu; Qun Dong; Hong Qiu; Chung-Wah Ma; Kan Ding
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-19
Journal Detail:
Title:  Carbohydrate research     Volume:  -     ISSN:  1873-426X     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-6-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Glycobiology and Glycochemistry Lab., Shanghai Institutes of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
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