| A homogalacturonan from the radix of Platycodon grandiflorum and the anti-angiogenesis activity of poly-/oligogalacturonic acids derived therefrom. | |
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MedLine Citation:
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PMID: 21700274 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A polysaccharide, PGA4-3b, with an average molecular weight of 8.9kDa estimated by high-performance gel-permeation chromatography (HPGPC), was isolated from radix of Platycodon grandiflorum (Jacq.) A. DC. Using monosaccharide analysis, methylation analysis and NMR spectroscopy, PGA4-3b was elucidated to be a linear poly-(1→4)-α-d-galactopyranosyluronic acid that contains no methyl ester groups. Partial acid hydrolysis of PGA4-3b yielded a series of poly- or oligogalacturonic acids with different degrees of polymerization (DP), that is, 4-3bde, 4-3bde-O-1, 4-3bde-O-2, 4-3bde-O-3, and 4-3bde-O-4. Cell tube formation inhibition tests with human microvascular endothelial cells (HMEC) for antiangiogenesis analysis showed that 4-3bde-O-1 and 4-3bde-O-2, the fractions with higher molecular weights, could inhibit tube formation, while the native PGA4-3b and low molecular weight fraction 4-3bde-O-3 and 4-3bde-O4 are ineffective. Moreover, 4-3bde-O-2 with DP 5-10 impaired cell tube formation in a dose-dependent way, suggesting its potential to be developed as an anti-angiogenesis drug. This is the first time oligogalacturonic acids are reported to show an anti-angiogenesis effect. |
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Authors:
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Yuxia Xu; Qun Dong; Hong Qiu; Chung-Wah Ma; Kan Ding |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-19 |
Journal Detail:
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Title: Carbohydrate research Volume: - ISSN: 1873-426X ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-6-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Glycobiology and Glycochemistry Lab., Shanghai Institutes of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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