Document Detail


The homeodomain protein PRH influences the differentiation of haematopoietic cells.
MedLine Citation:
PMID:  11077116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Haematopoiesis involves the differentiation of a self-renewing stem cell into all of the lineages found in circulating blood. Myb-Ets transformed chicken blastoderm cells (MEPs) have many of the characteristics of multipotent haematopoietic cells and represent a useful model system for the study of haematopoiesis. The proline-rich homeodomain protein (PRH) has previously been shown to be expressed in the haematopoietic compartment. In this report we show that PRH mRNA and protein levels are down regulated as MEPs differentiate along the myelomonocytic and erythrocytic lineages. In contrast, PRH mRNA and protein levels remain high as MEPs differentiate toward the thrombocytic lineage. Over-expression of full length PRH in MEPs inhibits their transformation and/or proliferation. However, the over-expression of N-terminally truncated PRH proteins results in normally proliferating cells that are predominantly differentiated along the myelomonocytic and eosinophilic lineages. These results suggest that PRH plays a role in the proliferation and differentiation of haematopoietic cells.
Authors:
P S Jayaraman; J Frampton; G Goodwin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  24     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-12     Completed Date:  2000-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1023-31     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Bristol, University Walk, BS8 1TD, Bristol, UK. sheela.jayaraman@bristol.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation / physiology*
Cell Division
Cell Line, Transformed
Down-Regulation
Gene Expression
Hematopoietic Stem Cells / cytology*,  metabolism
Homeodomain Proteins* / genetics,  metabolism
Humans
RNA, Messenger / metabolism
Transcription Factors
Chemical
Reg. No./Substance:
0/HHEX protein, human; 0/Homeodomain Proteins; 0/RNA, Messenger; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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