Document Detail

A hollow fibre dialysis system for the in vitro production of monoclonal antibodies replacing in vivo production in mice.
MedLine Citation:
PMID:  3582749     Owner:  NLM     Status:  MEDLINE    
Haemodialysis units have been used as bioreactors for the in vitro production of monoclonal antibodies. Mouse hybridomas were grown in the extra-capillary compartment of dialysis modules with a constant supply of protein-free medium through the hollow fibres. It was found that addition of serum to the media had no stimulatory effect on the production of monoclonal antibodies. For the determination of monoclonal antibodies a sol particle immunoassay (SPIA) has been used. Monoclonal antibodies were harvested from the extra-capillary compartment at two-week intervals over periods of several weeks at concentrations comparable to the harvests of ascitic fluids from mice. The production capacity of one haemodialysis unit is equivalent to that of at least 50 mice. This in vitro system enables a yearly production of 50 grams per unit of monoclonal antibodies of high purity and devoid of contaminants from mouse origin or serum. It has been shown that this system can be successfully used to produce a constant supply of monoclonal antibodies for a variety of applications, e.g. reagents for diagnostic tests and materials for immuno-chromatographic purification. As a consequence this in vitro production of monoclonal antibodies has replaced the many thousands of mice required for in vivo production.
O T Schönherr; P T van Gelder; P J van Hees; A M van Os; H W Roelofs
Related Documents :
3523489 - Production of a monoclonal antibody specific for seminomas and dysgerminomas.
1862939 - Monoclonal antibody to calmodulin: development, characterization, and comparison with p...
7066989 - Stage-specific antigens reacting with monoclonal antibodies against contact site a, a c...
6576989 - Rat al2, al3, al4 and al5 monoclonal antibodies bind to the common acute lymphoblastic ...
8634489 - Preclinical evaluation of spect imaging with 131i-labeled monoclonal antibody sz39 in n...
20568999 - Rat monoclonal antibody specific for the chromatin remodeling factor, chd1.
12855589 - Novel antimalarial antibodies highlight the importance of the antibody fc region in med...
14755329 - Achieving antigen-specific immune regulation.
7034809 - Immunologic classification of lymphocytic leukemias based on monoclonal antibody-define...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Developments in biological standardization     Volume:  66     ISSN:  0301-5149     ISO Abbreviation:  Dev. Biol. Stand.     Publication Date:  1987  
Date Detail:
Created Date:  1987-06-26     Completed Date:  1987-06-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0427140     Medline TA:  Dev Biol Stand     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  211-20     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal / biosynthesis*
Cell Division
Cells, Cultured
Culture Media
Hybridomas / metabolism*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Factors affecting monoclonal antibody production in culture.
Next Document:  Construction of a large scale membrane reactor system with different compartments for cells, medium ...