| hnRNP G elicits tumor-suppressive activity in part by upregulating the expression of Txnip. | |
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MedLine Citation:
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PMID: 18541147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heterogeneous nuclear ribonuclearproteins (hnRNPs) are nucleic acid-binding proteins and have critical roles in DNA repair, telomere regulation, and transcriptional gene regulation. Previously, we showed that hnRNP G has tumor-suppressive activity in human oral squamous cell carcinoma cells. Therefore, the identification of hnRNP G target genes is important for understanding the function of hnRNP G and its tumor-suppressive activity. In this study, we identify a known tumor suppressor gene, thioredoxin-interacting protein (Txnip) gene as a novel target of hnRNP G. Expression of Txnip is upregulated by wild-type (wt) hnRNP G but not by a suppression-defective mutant hnRNP G (K22R) in human squamous cell carcinoma. Wt hnRNP G binds and transactivates the Txnip promoter in vivo, whereas the K22R mutant does not. Furthermore, overexpression of Txnip alone in cancer cells leads to the inhibition of anchorage-independent growth and in vivo tumorigenicity in immunocompromised mice, suggesting a reversion of the transformation phenotype. These studies indicate that hnRNP G promotes the expression of Txnip and mediates its tumor-suppressive effect. |
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Authors:
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Ki-Hyuk Shin; Reuben H Kim; Roy H Kim; Mo K Kang; No-Hee Park |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-06-09 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 372 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-06-25 Completed Date: 2008-07-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 880-5 Citation Subset: IM |
Affiliation:
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School of Dentistry, University of California, CHS 43-033, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA. kshin@dentistry.ucla.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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genetics* Cell Line, Tumor Cell Proliferation Gene Expression Regulation, Neoplastic* Heterogeneous-Nuclear Ribonucleoproteins / genetics, metabolism* Humans Promoter Regions, Genetic Transcriptional Activation* Tumor Suppressor Proteins / genetics, metabolism* Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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DE14147/DE/NIDCR NIH HHS; DE15902/DE/NIDCR NIH HHS; R03 DE015902-01/DE/NIDCR NIH HHS; R03 DE015902-02/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Heterogeneous-Nuclear Ribonucleoproteins; 0/TXNIP protein, human; 0/Tumor Suppressor Proteins; 0/heterogeneous nuclear ribonucleoprotein G, human |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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