| The histochemistry of estrogen receptors. | |
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MedLine Citation:
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PMID: 7263294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Estrogen receptors in frozen section of the rat uterus were demonstrated by a radiolabeled ligand binding technique. The bound hormone was extracted with ethanol and measured by liquid scintillation. The binding of 3H-estradiol-17 beta at various molar concentrations was inhibited by a 100-fold excess of DES, and the bound 3H-estradiol resisted exhaustive washings at 4 degrees C for 16 h. These binding sites were not present in the sections of the spleen, and perhaps at a very low concentration in the myocardium. Thus their binding behavior and distribution pattern are consistent with those of specific estrogen receptors. The hydrophilic fluorescent estradiol conjugate, 17 beta-estradiol-6-CMO-BSA-FITC was found to be a highly effective competitor against binding of 3H-estradiol to its receptors in tissue sections, and is considered a useful histochemical reagent for localizing target cells with high concentrations of estrogen receptors. Estrogen receptor sites in frozen sections of human breast cancer were also measured by this radiolabeled ligand binding technique, and expressed in femtomoles of hormone bound per 1,000 cancer cells. The values were parallel to the histochemical findings in terms of percentage of the estrogen receptor-positive in the cancer cell population. |
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Authors:
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S H Lee |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histochemistry Volume: 71 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1981 |
Date Detail:
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Created Date: 1981-10-14 Completed Date: 1981-10-14 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 491-500 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding, Competitive Breast Neoplasms / analysis Estradiol / diagnostic use Female Histocytochemistry Humans Rats Receptors, Estrogen / analysis* Uterus / analysis |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Estrogen; 50-28-2/Estradiol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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