Document Detail

The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass.
MedLine Citation:
PMID:  19289085     Owner:  NLM     Status:  MEDLINE    
Outside cells of the preimplantation mouse embryo form the trophectoderm (TE), a process requiring the transcription factor Tead4. Here, we show that transcriptionally active Tead4 can induce Cdx2 and other trophoblast genes in parallel in embryonic stem cells. In embryos, the Tead4 coactivator protein Yap localizes to nuclei of outside cells, and modulation of Tead4 or Yap activity leads to changes in Cdx2 expression. In inside cells, Yap is phosphorylated and cytoplasmic, and this involves the Hippo signaling pathway component Lats. We propose that active Tead4 promotes TE development in outside cells, whereas Tead4 activity is suppressed in inside cells by cell contact- and Lats-mediated inhibition of nuclear Yap localization. Thus, differential signaling between inside and outside cell populations leads to changes in cell fate specification during TE formation.
Noriyuki Nishioka; Ken-ichi Inoue; Kenjiro Adachi; Hiroshi Kiyonari; Mitsunori Ota; Amy Ralston; Norikazu Yabuta; Shino Hirahara; Robert O Stephenson; Narumi Ogonuki; Ryosuke Makita; Hiroki Kurihara; Elizabeth M Morin-Kensicki; Hiroshi Nojima; Janet Rossant; Kazuki Nakao; Hitoshi Niwa; Hiroshi Sasaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental cell     Volume:  16     ISSN:  1878-1551     ISO Abbreviation:  Dev. Cell     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-17     Completed Date:  2009-04-02     Revised Date:  2011-04-22    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  398-410     Citation Subset:  IM    
Laboratory for Embryonic Induction, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.
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MeSH Terms
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Blastocyst Inner Cell Mass / metabolism*
Cells, Cultured
DNA-Binding Proteins / genetics,  metabolism*
Ectoderm / metabolism
Embryo Culture Techniques
Embryonic Stem Cells / metabolism
Gene Expression Regulation, Developmental
Homeodomain Proteins / genetics,  metabolism
Mice, Inbred C57BL
Mice, Inbred DBA
Mice, Mutant Strains
Mice, Transgenic
Models, Biological
Muscle Proteins / genetics,  metabolism*
Phosphoproteins / genetics,  metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Signal Transduction
Transcription Factors / genetics,  metabolism*
Trophoblasts / metabolism*
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Cdx2 protein, mouse; 0/DNA-Binding Proteins; 0/Homeodomain Proteins; 0/Muscle Proteins; 0/Phosphoproteins; 0/Tead4 protein, mouse; 0/Transcription Factors; 0/Yap protein, mouse; EC 2.7.1.-/Lats1 protein, mouse; EC Kinases
Comment In:
Dev Cell. 2009 Mar;16(3):321-2   [PMID:  19289076 ]
Dev Cell. 2011 Mar 15;20(3):e3   [PMID:  21411395 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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