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A highly sensitive "turn on" fluorescent sensor for the detection of human serum proteins based on the size exclusion of the polyacrylamide gel.
MedLine Citation:
PMID:  24150987     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A highly sensitive "turn-on" fluorescent sensor based on the size exclusion of the polyacrylamide gel was developed for the on-gels detection of human serum proteins after PAGE. The possible mechanism of this fluorescence sensor was illustrated and validated by utilizing five kinds of colloidal silver nanoparticles (CSNPs) with different particle size distribution and six kinds of polyacrylamide gels with different pore size. It was attributed to that silver nanoparticles (<5 nm in dimeter) had been selectively absorbed into the gel and formed the small silver nanoclusters, resulting in the red fluorescence. Using this new technique for the detection of human serum proteins after PAGE, a satisfactory sensitivity was achieved and some relatively low-abundance proteins (e.g., zinc-alpha-2-glycoprotein) which are the significant proteinic markers of certain diseases can be easily detected, but not with tranditional methods. Furthermore, it was also successfully applied to distinguish between serums from hepatoma patient and healthy people. As a new protein detection technique, the CSNPs-based "turn-on" fluorescent sensor offers a rapid, economic, low background and sensitive way for direct detection of human serum proteins, showing available potential and significance in the development of nanobiotechnology and proteome research. This article is protected by copyright. All rights reserved.
Authors:
Shenghao Xu; Pingping Liu; Xin Lu; Jing Zhang; Lingyun Huang; Wenhao Hua; Dacheng He; Jin Ouyang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-10-22
Journal Detail:
Title:  Electrophoresis     Volume:  -     ISSN:  1522-2683     ISO Abbreviation:  Electrophoresis     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
Affiliation:
Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, 100875, China.
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