| A highly carbapenem-resistant Pseudomonas aeruginosa isolate with a novel blaVIM-4/blaP1b integron overexpresses two efflux pumps and lacks OprD. | |
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MedLine Citation:
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PMID: 17483142 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: A Pseudomonas aeruginosa clinical isolate that exhibited high-level carbapenem resistance and produced metallo-beta-lactamase (MBL) was recovered from a Greek patient. This study was conducted to determine the underlying mechanisms that conferred the carbapenem resistance phenotype. METHODS: MICs were determined by Etest and Etest MBL. PCR assays were performed for identification of bla(VIM-type), other antibiotic resistance and efflux pump genes and mapping of class 1 integrons. Expression of efflux pump genes was quantified by real-time PCR. Nucleotide sequencing was used to determine the bla(VIM) allele. The location of the MBL allele was investigated by mating experiments, plasmid analysis and hybridization studies. RESULTS: The isolate was highly carbapenem-resistant (MICs of imipenem and meropenem were 512 and 128 mg/L, respectively) and multidrug-resistant. It harboured the beta-lactamase genes bla(VIM-4) and bla(P1b) in a novel class 1 integron named InV4P1, and a second integron with aac(6')-Ib and bla(OXA-35) gene cassettes. The isolate was deficient in porin OprD and overexpressed efflux pumps MexAB-OprM and MexXY-OprM. Conjugation experiments failed to detect transferable MBL determinants, plasmids were not visualized and bla(VIM) was detected by PCR in the chromosomal band. CONCLUSIONS: Multiple carbapenem resistance mechanisms are demonstrated to coexist in a single P. aeruginosa isolate and might confer the high-level carbapenem resistance. |
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Authors:
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Maria Maniati; Alexandros Ikonomidis; Paraskevi Mantzana; Alexandros Daponte; Antonios N Maniatis; Spyros Pournaras |
Publication Detail:
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Type: Journal Article Date: 2007-05-04 |
Journal Detail:
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Title: The Journal of antimicrobial chemotherapy Volume: 60 ISSN: 0305-7453 ISO Abbreviation: J. Antimicrob. Chemother. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-06-19 Completed Date: 2007-09-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7513617 Medline TA: J Antimicrob Chemother Country: England |
Other Details:
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Languages: eng Pagination: 132-5 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Anti-Bacterial Agents / pharmacology* Bacterial Proteins / genetics, metabolism Carbapenems / pharmacology* Drug Resistance, Multiple, Bacterial / genetics* Female Greece Humans Integrons / genetics* Microbial Sensitivity Tests Porins / metabolism Pseudomonas Infections / microbiology Pseudomonas aeruginosa / drug effects*, enzymology, genetics, isolation & purification beta-Lactam Resistance beta-Lactamases / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Carbapenems; 0/Porins; 148412-32-2/OprD protein, Pseudomonas aeruginosa; EC 3.5.2.6/beta-Lactamases; EC 3.5.2.6/beta-lactamase bla(VIM-4), Pseudomonas aeruginosa |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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