Document Detail


The highest levels of purine catabolic enzymes in mice are present in the proximal small intestine.
MedLine Citation:
PMID:  8226898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies on the tissue distribution and developmental regulation of adenosine deaminase (ADA) activity in mice show that very high ADA levels exist in the murine alimentary tract (tongue, esophagus, forestomach, proximal small intestine) and at the fetal-maternal interface. To understand the role of ADA in these tissues, we measured the levels of three other enzymes involved in purine catabolism, purine nucleoside phosphorylase (PNP), guanine deaminase (GDA), and xanthine dehydrogenase (XDH), to see how their levels correlated with ADA activity. Our results show that the highest level of PNP, GDA, and XDH is present in the proximal small intestine. Levels of these purine catabolic enzymes are much lower in the tongue, esophagus, forestomach, and fetal-maternal interface in marked contrast to ADA distribution. We also determined mRNA levels encoding PNP, XDH, and ADA in a variety of tissues. Tissue-specific differences in PNP, XDH, and ADA activity correlated with RNA abundance, indicating that the regulation of gene expression is at the level of mRNA production. Thus, ADA is part of a purine catabolic pathway leading to the production of uric acid that is present at the highest known level in the proximal small intestine. ADA may have additional roles in other tissues.
Authors:
K A Mohamedali; O M Guicherit; R E Kellems; F B Rudolph
Related Documents :
12928488 - Pten: tumour suppressor, multifunctional growth regulator and more.
24291478 - High-frequency stimulation of the globus pallidus interna nucleus modulates gfrα1 gene ...
22445828 - Gene transcripts in spermatozoa: markers of male infertility.
9428408 - Spmyb functions as an intramodular repressor to regulate spatial expression of cyiiia i...
23935508 - Rbm5 is a male germ cell splicing factor and is required for spermatid differentiation ...
10974658 - Tissue-specific regulation of gastrin-releasing peptide synthesis, storage and secretio...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  268     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-11-29     Completed Date:  1993-11-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23728-33     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Deaminase / genetics,  metabolism*
Animals
Gene Expression
Guanine Deaminase / genetics,  metabolism*
Intestine, Small / enzymology*
Mice
Purine-Nucleoside Phosphorylase / genetics,  metabolism*
Purines / metabolism*
RNA, Messenger / genetics
Uric Acid / metabolism
Xanthine Dehydrogenase / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
CA14030/CA/NCI NIH HHS; DK46207/DK/NIDDK NIH HHS; GM30204/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Purines; 0/RNA, Messenger; 69-93-2/Uric Acid; EC 1.17.1.4/Xanthine Dehydrogenase; EC 2.4.2.1/Purine-Nucleoside Phosphorylase; EC 3.5.4.3/Guanine Deaminase; EC 3.5.4.4/Adenosine Deaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In vivo and in vitro phosphorylation of the T lymphocyte type n (Kv1.3) potassium channel.
Next Document:  Primary structure and muscle-specific expression of the 50-kDa dystrophin-associated glycoprotein (a...