Document Detail

The highest levels of purine catabolic enzymes in mice are present in the proximal small intestine.
MedLine Citation:
PMID:  8226898     Owner:  NLM     Status:  MEDLINE    
Recent studies on the tissue distribution and developmental regulation of adenosine deaminase (ADA) activity in mice show that very high ADA levels exist in the murine alimentary tract (tongue, esophagus, forestomach, proximal small intestine) and at the fetal-maternal interface. To understand the role of ADA in these tissues, we measured the levels of three other enzymes involved in purine catabolism, purine nucleoside phosphorylase (PNP), guanine deaminase (GDA), and xanthine dehydrogenase (XDH), to see how their levels correlated with ADA activity. Our results show that the highest level of PNP, GDA, and XDH is present in the proximal small intestine. Levels of these purine catabolic enzymes are much lower in the tongue, esophagus, forestomach, and fetal-maternal interface in marked contrast to ADA distribution. We also determined mRNA levels encoding PNP, XDH, and ADA in a variety of tissues. Tissue-specific differences in PNP, XDH, and ADA activity correlated with RNA abundance, indicating that the regulation of gene expression is at the level of mRNA production. Thus, ADA is part of a purine catabolic pathway leading to the production of uric acid that is present at the highest known level in the proximal small intestine. ADA may have additional roles in other tissues.
K A Mohamedali; O M Guicherit; R E Kellems; F B Rudolph
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  268     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-11-29     Completed Date:  1993-11-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  23728-33     Citation Subset:  IM    
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005.
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MeSH Terms
Adenosine Deaminase / genetics,  metabolism*
Gene Expression
Guanine Deaminase / genetics,  metabolism*
Intestine, Small / enzymology*
Purine-Nucleoside Phosphorylase / genetics,  metabolism*
Purines / metabolism*
RNA, Messenger / genetics
Uric Acid / metabolism
Xanthine Dehydrogenase / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Purines; 0/RNA, Messenger; 69-93-2/Uric Acid; EC Dehydrogenase; EC Phosphorylase; EC Deaminase; EC Deaminase

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