| A high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes. | |
| | |
MedLine Citation:
|
PMID: 3036986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A counterflow centrifugal elutriation procedure for preparing human granulocytes is described. This method permits isolation of 1-2 X 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. These cells are 99% +/- 1% pure and 95-99% viable. When compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. Other responses including receptor-mediated uptake of chemoattractant were not significantly different from those of conventionally prepared cells after incubation at 37 degrees C prior to stimulation. The population of elutriated granulocytes, however, was significantly more homogeneous and pure than cells prepared conventionally. |
| | |
Authors:
|
J O Tolley; G M Omann; A J Jesaitis |
Related Documents
:
|
11121606 - Use of substrate responsive-direct viable counts to visualize naphthalene degrading bac... 4295346 - Methods for the determination of adipose cell size in man and animals. 3519556 - Variables in estimation of adipocyte size and number with a particle counter. 20462626 - The impact of differing cell and algogenic organic matter (aom) characteristics on the ... 1993596 - Flow cytometry measurements of the dna content of corneal epithelial cells during wound... 6331526 - Transformation-associated changes in nuclear-coded mitochondrial proteins in 3t3 cells ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of leukocyte biology Volume: 42 ISSN: 0741-5400 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 1987 Jul |
Date Detail:
|
Created Date: 1987-08-21 Completed Date: 1987-08-21 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 43-50 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Separation
/
methods* Cell Survival Centrifugation Granulocytes / cytology* Humans Kinetics Microscopy, Electron, Scanning N-Formylmethionine Leucyl-Phenylalanine / blood Neutrophils / cytology Superoxides / blood Tetradecanoylphorbol Acetate / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
|
A117354//PHS HHS; HL16411/HL/NHLBI NIH HHS; RROO833/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
11062-77-4/Superoxides; 16561-29-8/Tetradecanoylphorbol Acetate; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Volume dependent polymorphonuclear leukocytes fractions isolated by counterflow centrifugal elutriat...
Next Document: Defective production of reactive oxygen intermediates by tumor-associated macrophages exposed to pho...