Document Detail


A high-resolution view of genome-wide pneumococcal transformation.
MedLine Citation:
PMID:  22719250     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transformation is an important mechanism of microbial evolution through which bacteria have been observed to rapidly adapt in response to clinical interventions; examples include facilitating vaccine evasion and the development of penicillin resistance in the major respiratory pathogen Streptococcus pneumoniae. To characterise the process in detail, the genomes of 124 S. pneumoniae isolates produced through in vitro transformation were sequenced and recombination events detected. Those recombinations importing the selected marker were independent of unselected events elsewhere in the genome, the positions of which were not significantly affected by local sequence similarity between donor and recipient or mismatch repair processes. However, both types of recombinations were sometimes mosaic, with multiple non-contiguous segments originating from the same molecule of donor DNA. The lengths of the unselected events were exponentially distributed with a mean of 2.3 kb, implying that recombinations are stochastically resolved with a fixed per base probability of 4.4×10(-4) bp(-1). This distribution of recombination sizes, coupled with an observed under representation of large insertions within transferred sequence, suggests transformation has the potential to reduce the size of bacterial genomes, and is unlikely to act as an efficient mechanism for the uptake of accessory genomic loci.
Authors:
Nicholas J Croucher; Simon R Harris; Lars Barquist; Julian Parkhill; Stephen D Bentley
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-14
Journal Detail:
Title:  PLoS pathogens     Volume:  8     ISSN:  1553-7374     ISO Abbreviation:  PLoS Pathog.     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-21     Completed Date:  2012-11-09     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101238921     Medline TA:  PLoS Pathog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e1002745     Citation Subset:  IM    
Affiliation:
Pathogen Genomics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom. nc3@sanger.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Base Sequence
Biological Evolution*
DNA, Bacterial / genetics*
Genome, Bacterial / genetics*
Molecular Sequence Data
Polymorphism, Single Nucleotide
Recombination, Genetic
Streptococcus pneumoniae / genetics*
Transformation, Bacterial / genetics*
Grant Support
ID/Acronym/Agency:
098051//Wellcome Trust
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Salmonella deubiquitinase SseL inhibits selective autophagy of cytosolic aggregates.
Next Document:  Crystal structures reveal the multi-ligand binding mechanism of Staphylococcus aureus ClfB.