| A high pressure liquid chromatographic method for the determination of tetracyclines in blood and organs of experimental animals. | |
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MedLine Citation:
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PMID: 543876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high-pressure liquid chromatographic method has been developed for the quantitative analysis of tetracyclines from biological material. Tetracyclines can be extracted from serum or organ homogenates by means of acetonitrile containing buffer. The extract is chromatographed on a reversed-phase column and the tetracyclines are monitored spectrophotometerically at 357 nm. Tetracyclines, pyrrolidinomethyl-tetracycline, 4-epitetracycline, and doxycycline are thus separated within 8 min. The lowest concentration for the intact molecule detectable are 0.2 micrograms/ml serum or blood and 0.4 micrograms/g organ. In a comparative study the new extraction procedure and the chromatographic analysis yielded the same results as a microbiological method. |
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Authors:
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R Böcker; C J Estler |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Arzneimittel-Forschung Volume: 29 ISSN: 0004-4172 ISO Abbreviation: Arzneimittelforschung Publication Date: 1979 |
Date Detail:
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Created Date: 1980-06-16 Completed Date: 1980-06-16 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372660 Medline TA: Arzneimittelforschung Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 1690-3 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid Male Methods Mice Organ Size Rolitetracycline / analysis Tetracyclines / analysis*, blood Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Tetracyclines; 751-97-3/Rolitetracycline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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