Document Detail

A high pressure cell for simultaneous osmotic pressure and x-ray diffraction measurements.
MedLine Citation:
PMID:  19334952     Owner:  NLM     Status:  MEDLINE    
In this paper, we report on a novel osmotic cell, developed to simultaneously subject a sample to osmotic stress and measure structural changes by small angle x-ray diffraction. The osmotic cell offers many advantages over more conventional methods of osmotically stressing soft materials to measure their structural response. In particular, a full osmotic analysis can be performed with a single small sample (25 microl). This reduces sample handling and the associated systematic errors, as well as enabling tight control and monitoring of the thermodynamic environment during osmosis, thereby increasing measurement precision. The cell design enables control of osmotic pressure to +/-0.04 bar over a pressure range of 1-100 bar, and temperature control to +/-0.05 degrees C. Under these conditions, the lattice spacing in lyotropic structures was resolved to better than +/-0.005 A. Using the osmotic cell, we demonstrate good agreement with previous conventional measurements on the energy of dehydrating the fluid lamellar phase of dioleoylphosphatidylcholine in water.
Béatrice L L E Gauthé; Andrew J Heron; John M Seddon; Oscar Ces; Richard H Templer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Review of scientific instruments     Volume:  80     ISSN:  1089-7623     ISO Abbreviation:  Rev Sci Instrum     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-04-01     Completed Date:  2009-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0405571     Medline TA:  Rev Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  035107     Citation Subset:  IM    
Department of Chemistry and Chemical Biology Centre, Imperial College London, London SW7 2AZ, United Kingdom.
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MeSH Terms
Computer-Aided Design
Equipment Design
Equipment Failure Analysis
Lipids / chemistry*
Membranes, Artificial*
Osmotic Pressure
Reproducibility of Results
Rheology / instrumentation*
Sensitivity and Specificity
Specimen Handling / instrumentation*
X-Ray Diffraction / instrumentation*
Grant Support
28/B15299//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Lipids; 0/Membranes, Artificial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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