Document Detail


A high-performance liquid chromatographic-tandem mass spectrometric method for the determination of cethromycin (ABT-773) in human plasma, bronchoalveolar lavage fluid, and alveolar cells.
MedLine Citation:
PMID:  14596787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is developed for the specific and sensitive determination of cethromycin concentrations in plasma, bronchoalveolar lavage (BAL), and alveolar cells (AC), using a high-performance liquid chromatographic-tandem mass spectrometry (MS) method. The mobile phase consists of 50% acetonitrile-0.05% acetic acid-5mM ammonium acetate; the column used is a C(8) reversed-phase stationary phase. The preparation of samples requires a solvent extraction step. The retention times for cethromycin and the internal standard are approximately 2.0 and 2.7 min, respectively, with a total run time of 3.5 min. Detection is carried out using electrospray MS in a multiple reaction monitor mode. The detection limits for cethromycin are 1 ng/mL for plasma and 0.2 ng/mL for BAL supernatants and AC suspensions. The assay has excellent performance characteristics and has been used to support a study of the intrapulmonary pharmacokinetics of cethromycin in human subjects.
Authors:
Qiulei Ren; John E Conte; Elisabeth Zurlinden; Emil T Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  41     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-11-04     Completed Date:  2004-01-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  494-9     Citation Subset:  IM    
Affiliation:
Department of Biopharmaceutical Sciences, University of California, San Francisco, San Francisco, CA 94117, USA.
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MeSH Terms
Descriptor/Qualifier:
Bronchoalveolar Lavage Fluid / chemistry*
Chromatography, High Pressure Liquid / methods*
Erythromycin / analogs & derivatives*,  analysis*,  blood
Humans
Ketolides*
Pulmonary Alveoli / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization / methods*
Chemical
Reg. No./Substance:
0/Ketolides; 0/cethromycin; 114-07-8/Erythromycin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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