| The high mobility group I/Y protein is hypophosphorylated in endoreduplicating maize endosperm cells and is involved in alleviating histone H1-mediated transcriptional repression. | |
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MedLine Citation:
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PMID: 10864924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During maize endosperm development, cells shift from a mitotic cycle to endoreduplication, driving the massive synthesis of storage proteins (zeins) and starch. In this developmental context, we studied changes in expression levels of histone H1 and high mobility group I/Y (HMG-I/Y), two chromatin architectural proteins that are known to affect gene transcription. Almost no change was found in the level of histone H1 during endosperm development, despite a dramatic increase in DNA content (endoreduplication); hence, the histone H1/DNA ratio decreased substantially. Concurrently with a reduction in the Cdc2 kinase activity at the shift to endoreduplication, significant changes were found in the level and mobility of the HMG-I/Y protein; the faster migrating forms were, at least partly, hypophosphorylated. Purified maize HMG-I/Y protein was found to be phophorylated in vitro by the Cdc2 kinase and bound efficiently to the gamma-zein promoter AT-rich tract (gammaZ-AT). Using an in vitro transcription assay, we demonstrated the capability of the maize HMG-I/Y protein to relieve the inhibitory effect exerted by histone H1 on templates containing the gammaZ-AT sequence. These data suggest that during maize endosperm development transcription and perhaps replication are controlled, at least partly, by the activity of the Cdc2 kinase and the interplay between histone H1 and HMG-I/Y proteins. |
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Authors:
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J Zhao; G Grafi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 275 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-03 Completed Date: 2000-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 27494-9 Citation Subset: IM |
Affiliation:
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Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA Primers HMGA1a Protein* High Mobility Group Proteins / metabolism* Histones / metabolism* Phosphorylation Plant Proteins / metabolism* Repressor Proteins / metabolism* Transcription, Genetic* Zea mays / cytology, growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/HMGA1a Protein, plant; 0/High Mobility Group Proteins; 0/Histones; 0/Plant Proteins; 0/Repressor Proteins; 124544-67-8/HMGA1a Protein |
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