Document Detail


A high melting structure in DNA distinguishes phases of the cell cycle.
MedLine Citation:
PMID:  3391238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differential scanning microcalorimetry of the nuclei of dividing CHO cells revealed DNA structures that showed structural transitions at 60, 76, 88, and 105 degrees C (transitions I to IV, respectively). In cultures synchronized by isoleucine deprivation the enthalpies of transitions I and II were rather constant throughout the cell cycle. While the sum of the enthalpies of III and IV was nearly constant, the ratio of IV to III varied substantially from one phase of the cycle to another. A high IV:III ratio of 6 characterized G1 while S phase gave a IV:III ratio of about 2. Cells containing metaphase chromosomes also showed a IV:III ratio near 2. The IV:III ratio for CHO cells showed a progressive decrease as the cells were maintained in isoleucine-free medium from 0 to 6 days.
Authors:
G A Rice; N A Touchette; A N Creager; J Goldberg; R D Cole
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  177     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-17     Completed Date:  1988-08-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of California, Berkeley 94720.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calorimetry, Differential Scanning
Cell Cycle*
Cricetinae
DNA* / biosynthesis
Female
Hot Temperature*
Interphase
Isoleucine / physiology
Kinetics
Mitosis
Ovary
Thermodynamics
Grant Support
ID/Acronym/Agency:
ES07075/ES/NIEHS NIH HHS; GM07232/GM/NIGMS NIH HHS; GM20338/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
73-32-5/Isoleucine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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