| A high expression of heme oxygenase-1 in the liver of LEC rats at the stage of hepatoma: the possible implication of induction in uninvolved tissue. | |
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MedLine Citation:
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PMID: 9684983 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined changes in the expression of heme oxygenase-1 (HO-1), an inducible isoform and HO-2, a constitutive isoform, in the liver of Long-Evans with a Cinnamon-like color (LEC) rat, a mutant strain which spontaneously develops acute hepatitis and hepatoma. HO-1 expression was highly enhanced in the LEC rat livers with jaundice, and then decreased slightly, but overall remained at a higher level than in the Long-Evans with Agouti color (LEA) control rats, as judged by Northern blotting analysis of the whole liver extract. The high expression of HO-1 in the LEC rat liver was, however, not due to the actual cancer lesion but, rather, due to the surrounding uninvolved tissues including hepatocytes. Immunohistochemical analysis also supported this conclusion. Among normal tissues, the expression of HO-1 but not HO-2 was high in only the spleen of both LEC and LEA rats. The high expression observed in the stage of acute hepatitis and hepatoma stages in the LEC rat is probably due to the oxidative stress caused by the accumulation of free copper and free iron levels which has been reported earlier by our group (Suzuki et al., Carcinogenesis, 1993, 14, 1881-1884 and Koizumi et al., Free Radical Research, in press) as well as by free heme levels. The inflammatory cytokines produced by the surrounding tissue at the hepatoma stage would also be expected to play a role in the induction mechanism. The physiological relevance of HO-1 induction might be an adaptive response to oxidative stress and vasodilatory effect of carbon monoxide on sinusoidal circulation. |
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Authors:
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A Matsumoto; R Hanayama; M Nakamura; K Suzuki; J Fujii; H Tatsumi; N Taniguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 28 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-10-19 Completed Date: 1998-10-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 383-91 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Osaka University Medical School, Suita, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Carcinoma, Hepatocellular / enzymology* Gene Expression Regulation, Developmental Heme Oxygenase (Decyclizing) / genetics, metabolism* Heme Oxygenase-1 Immunohistochemistry Jaundice / enzymology Liver / metabolism* Liver Neoplasms / enzymology Oxidative Stress RNA, Messenger / metabolism Rats Rats, Mutant Strains Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 1.14.99.3/Heme Oxygenase (Decyclizing); EC 1.14.99.3/Heme Oxygenase-1; EC 1.14.99.3/heme oxygenase-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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