| A high cell density fermentation strategy to produce recombinant malarial antigen in E. coli. | |
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MedLine Citation:
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PMID: 15672234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high cell density cultivation method was developed to produce recombinant PvRII, a malaria vaccine candidate, in E. coli for use in vaccine studies. Cells were grown in completely defined media and glucose was fed to achieve a specific growth rate of 0.12 h(-1) until cells reached 55 g dry wt l(-1). Culture was then induced with 1 mM: IPTG and cells were further grown for 4 h to reach 85 g dry wt l(-1) at 0.1 h(-1). Recombinant PvRII was purified from inclusion bodies under denaturing conditions using metal affinity chromatography which yielded 10 mg PvRII g(-1) dry wt. After refolding, PvRII was greater than 98% pure, homogeneous and functionally active in that it specifically bound Duffy positive human red cells. |
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Authors:
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Syed Shams Yazdani; Ahmad Rushdi Shakri; Chetan E Chitnis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology letters Volume: 26 ISSN: 0141-5492 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2005-01-26 Completed Date: 2005-07-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1891-5 Citation Subset: IM |
Affiliation:
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Malaria Research Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, New Delhi 110067, India. shams@icgeb.res.in |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Protozoan
/
analysis,
biosynthesis*,
chemistry,
genetics* Cell Count Cell Culture Techniques / methods* Cell Proliferation Escherichia coli / genetics*, metabolism* Fermentation / physiology Gene Expression Regulation, Bacterial / physiology Genetic Enhancement / methods Protein Engineering / methods* Protozoan Proteins / analysis, biosynthesis*, chemistry, genetics* Receptors, Cell Surface / analysis, biosynthesis*, chemistry, genetics* Recombinant Proteins / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Protozoan; 0/Duffy antigen binding protein, Plasmodium; 0/Protozoan Proteins; 0/Receptors, Cell Surface; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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