| The high alkaline fraction on isoelectric focusing of cerebrospinal fluid is cystatin C. | |
| | |
MedLine Citation:
|
PMID: 9600685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A high alkaline fraction with a pI of 9.2 is sometimes seen on isoelectric focusing patterns of cerebrospinal fluid. The appearance of this fraction mainly depends on the type of concentrators used to prepare the cerebrospinal fluid samples, prior to isoelectric focusing. The amino acid sequence of the high alkaline fraction showed sequence identity to cystatin C, a cysteine protease inhibitor with a pI of 9.2-9.3 and a molecular mass of 13.4 kDa. In addition, on Western blot the high alkaline fraction was recognized by an antibody, directed against cystatin C. Taken together, the present findings demonstrate that the high alkaline fraction is cystatin C. |
| | |
Authors:
|
F W Roelandse; R Amons; E P ter Braak; R Nieuwland; J van Loon; J H Souverijn |
Related Documents
:
|
8673075 - Catabolism of dopamine and 5-hydroxytryptamine by monoamine oxidase in the ixodid tick,... 9150615 - Cerebrospinal fluid neuropeptides and monoaminergic transmitters in patients with trige... 16998855 - Amino acids in oviduct and uterine fluid and blood plasma during the estrous cycle in t... 6625645 - Polyamine levels in normal human skin. a comparative study of pure epidermis, pure derm... 18953095 - Luminal hydrochloric acid stimulates rapid transepithelial ion fluxes in rodent esophag... 18964045 - Determination of phenothiazines by charge-transfer complex formation with chloranilic a... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of the neurological sciences Volume: 157 ISSN: 0022-510X ISO Abbreviation: J. Neurol. Sci. Publication Date: 1998 Apr |
Date Detail:
|
Created Date: 1998-12-09 Completed Date: 1998-12-09 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0375403 Medline TA: J Neurol Sci Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 105-8 Citation Subset: IM |
Affiliation:
|
Department of Clinical Chemistry, Leiden University Medical Centre, Netherlands. roelandse@rullf2.medfac.leidenuniv.nl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Cerebrospinal Fluid Proteins / cerebrospinal fluid, isolation & purification* Cystatin C Cystatins / cerebrospinal fluid, isolation & purification* Humans Isoelectric Focusing / methods |
| Chemical | |
Reg. No./Substance:
|
0/CST3 protein, human; 0/Cerebrospinal Fluid Proteins; 0/Cystatin C; 0/Cystatins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: An atypical case of adult Moyamoya disease with initial onset of brain stem ischemia.
Next Document: HTLV-I-associated myelopathy associated with multi-organ inflammatory disease: a case report.