| The heterogeneity of cell subtypes from a primary culture of human amniotic fluid. | |
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MedLine Citation:
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PMID: 20499195 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heterogeneous human amniotic fluid contains various cell types. Herein, we report on the possibility of simultaneously isolating three subtypes of cells from one primary culture. Using a stainless steel instrument named a colony poculum, two of the three cell subtypes could be efficiently cultured, and these were further characterized. The results indicated that these two cell subtypes had different morphologies and were characterized by different cell marker expression profiles, including the differential expression of CD105, CD117 and EBAF. Furthermore, their gene expression array data revealed their different gene expression profiles. Although both cell types expressed several embryonic stem cell-specific markers, they were non-tumorigenic in vivo. This paper not only provides new insight into the heterogeneity of human amniotic fluid, it also presents a simple yet efficient cell isolation method. These results will contribute to the thorough investigation of the properties and potential future applications of human amniotic fluid-derived cells. |
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Authors:
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Shengli Zhang; Hongquan Geng; Hua Xie; Qiquan Wu; Xiaorong Ma; Junmei Zhou; Fang Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-24 |
Journal Detail:
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Title: Cellular & molecular biology letters Volume: 15 ISSN: 1689-1392 ISO Abbreviation: Cell. Mol. Biol. Lett. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-06-21 Completed Date: 2010-09-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607427 Medline TA: Cell Mol Biol Lett Country: Poland |
Other Details:
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Languages: eng Pagination: 424-39 Citation Subset: IM |
Affiliation:
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Department of Urology, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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cytology* Antigens, CD / metabolism Biological Markers / metabolism Cells, Cultured Flow Cytometry Gene Expression Profiling Humans Karyotyping Left-Right Determination Factors / metabolism Proto-Oncogene Proteins c-kit / metabolism Receptors, Cell Surface / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Biological Markers; 0/ENG protein, human; 0/LEFTY2 protein, human; 0/Left-Right Determination Factors; 0/Receptors, Cell Surface; EC 2.7.10.1/Proto-Oncogene Proteins c-kit |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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