| The herpes simplex virus receptor nectin-1 is down-regulated after trans-interaction with glycoprotein D. | |
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MedLine Citation:
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PMID: 18076965 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During herpes simplex virus (HSV) entry, membrane fusion occurs either on the cell surface or after virus endocytosis. In both cases, binding of glycoprotein D (gD) to a receptor such as nectin-1 or HVEM is required. In this study, we co-cultured cells expressing gD with nectin-1 expressing cells to investigate the effects of gD on nectin-1 at cell contacts. After overnight co-cultures with gD expressing cells, there was a down-regulation of nectin-1 in B78H1-C10, SY5Y, A431 and HeLa cells, which HSV enters by endocytosis. In contrast, on Vero cells, which HSV enters at the plasma membrane, nectin-1 was not down-regulated. Further analysis of B78H1-derived cells showed that nectin-1 down-regulation corresponds to the ability of gD to bind nectin-1 and is achieved by internalization and low-pH-dependent degradation of nectin-1. Moreover, gD is necessary for virion internalization in B78H1 cells expressing nectin-1. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry. |
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Authors:
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Katie M Stiles; Richard S B Milne; Gary H Cohen; Roselyn J Eisenberg; Claude Krummenacher |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-02-20 |
Journal Detail:
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Title: Virology Volume: 373 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-17 Completed Date: 2008-04-22 Revised Date: 2013-04-11 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 98-111 Citation Subset: IM |
Affiliation:
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Department of Microbiology, School of Dental Medicine University of Pennsylvania, Philadelphia, PA 19104, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Molecules / genetics, metabolism* Cell Line Cercopithecus aethiops Down-Regulation* Endocytosis HeLa Cells Herpesvirus 1, Human / genetics, metabolism, pathogenicity* Humans Mice Receptors, Virus / genetics, metabolism* Transfection Vero Cells Viral Envelope Proteins / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI-056045/AI/NIAID NIH HHS; AI-18289/AI/NIAID NIH HHS; NS-36731/NS/NINDS NIH HHS; R01 AI056045-04/AI/NIAID NIH HHS; R01 AI076231-15/AI/NIAID NIH HHS; R01 NS036731-10/NS/NINDS NIH HHS; R37 AI018289/AI/NIAID NIH HHS; R37 AI018289-26/AI/NIAID NIH HHS; T32 GM007229-32/GM/NIGMS NIH HHS; T32-GM07229/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Adhesion Molecules; 0/Receptors, Virus; 0/Viral Envelope Proteins; 0/glycoprotein D, Human herpesvirus 1; 0/nectins |
| Comments/Corrections | |
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