Document Detail


The herpes simplex virus receptor nectin-1 is down-regulated after trans-interaction with glycoprotein D.
MedLine Citation:
PMID:  18076965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During herpes simplex virus (HSV) entry, membrane fusion occurs either on the cell surface or after virus endocytosis. In both cases, binding of glycoprotein D (gD) to a receptor such as nectin-1 or HVEM is required. In this study, we co-cultured cells expressing gD with nectin-1 expressing cells to investigate the effects of gD on nectin-1 at cell contacts. After overnight co-cultures with gD expressing cells, there was a down-regulation of nectin-1 in B78H1-C10, SY5Y, A431 and HeLa cells, which HSV enters by endocytosis. In contrast, on Vero cells, which HSV enters at the plasma membrane, nectin-1 was not down-regulated. Further analysis of B78H1-derived cells showed that nectin-1 down-regulation corresponds to the ability of gD to bind nectin-1 and is achieved by internalization and low-pH-dependent degradation of nectin-1. Moreover, gD is necessary for virion internalization in B78H1 cells expressing nectin-1. These data suggest that the determinants of gD-mediated internalization of nectin-1 may direct HSV to an endocytic pathway during entry.
Authors:
Katie M Stiles; Richard S B Milne; Gary H Cohen; Roselyn J Eisenberg; Claude Krummenacher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-20
Journal Detail:
Title:  Virology     Volume:  373     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-17     Completed Date:  2008-04-22     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  98-111     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion Molecules / genetics,  metabolism*
Cell Line
Cercopithecus aethiops
Down-Regulation*
Endocytosis
HeLa Cells
Herpesvirus 1, Human / genetics,  metabolism,  pathogenicity*
Humans
Mice
Receptors, Virus / genetics,  metabolism*
Transfection
Vero Cells
Viral Envelope Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
AI-056045/AI/NIAID NIH HHS; AI-18289/AI/NIAID NIH HHS; NS-36731/NS/NINDS NIH HHS; R01 AI056045/AI/NIAID NIH HHS; R01 AI056045-04/AI/NIAID NIH HHS; R01 AI076231/AI/NIAID NIH HHS; R01 NS036731/NS/NINDS NIH HHS; R01 NS036731-10/NS/NINDS NIH HHS; R37 AI018289/AI/NIAID NIH HHS; R37 AI018289-26/AI/NIAID NIH HHS; T32 GM007229/GM/NIGMS NIH HHS; T32 GM007229-32/GM/NIGMS NIH HHS; T32-GM07229/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Receptors, Virus; 0/Viral Envelope Proteins; 0/glycoprotein D, Human herpesvirus 1; 0/nectins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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