Document Detail

The hemolymph of caterpillars Spodoptera littoralis: physico-chemical properties and ionic composition compared to culture media.
MedLine Citation:
PMID:  15560259     Owner:  NLM     Status:  MEDLINE    
In this paper, we determined some physico-chemical properties like osmotic pressure, pH and electrical conductivity of the hemolymph from caterpillars of Spodoptera littoralis (Lepidoptera: Noctuidae) during the last larval instar. It was of interest that we observed an increase in osmotic pressure with the increase in age in the last instar that may concur with the start of histolysis at metamorphosis. These physicochemical properties were then compared to those of Grace's and modified Grace's tissue culture medium. In addition, concentrations of the cations Na, K, Ca and Mg, and the anions Cl, NO3, PO4 and SO4 were determined in the insect hemolymph of S. littoralis. The cations K and Mg reached high values with a percent of about 52% of the total amount of cations. The concentration of sodium was low. The total sum of the anions consisted about 56 meq/1, and this allows to neutralise about 45 % of the total cations.
G Smagghe; T Van Leeuwen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Communications in agricultural and applied biological sciences     Volume:  69     ISSN:  1379-1176     ISO Abbreviation:  Commun. Agric. Appl. Biol. Sci.     Publication Date:  2004  
Date Detail:
Created Date:  2004-11-24     Completed Date:  2005-03-07     Revised Date:  2006-12-14    
Medline Journal Info:
Nlm Unique ID:  101200320     Medline TA:  Commun Agric Appl Biol Sci     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  15-22     Citation Subset:  IM    
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
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MeSH Terms
Anions / analysis
Cations / analysis
Culture Media
Electric Conductivity
Hemolymph / chemistry*,  physiology
Hydrogen-Ion Concentration
Spodoptera / growth & development
Reg. No./Substance:
0/Anions; 0/Cations; 0/Culture Media

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