| A heat-stable component of Bartonella henselae upregulates intercellular adhesion molecule-1 expression on vascular endothelial cells. | |
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MedLine Citation:
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PMID: 11967118 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bartonella henselae upregulated the expression of intercellular adhesion molecule-1 (ICAM-1) on human umbilical vein endothelial cells (HUVECs). The induction level of ICAM-1 depended on the inoculation bacterial dose. ICAM-1 expression began increasing 4 h after infection and reached a sustained peak beginning at 12 h after B. henselae infection; this time course was similar to that of lipopolysaccharide (LPS) of Escherichia coli. The stimulatory effect was abolished when live B. henselae were separated from HUVECs by a filter membrane. The nonpiliated strain, which is unable to invade endothelial cells, induced ICAM-1 expression to the same extent as the piliated strain. Inactivation of B. henselae by ultraviolet (UV) irradiation, heat (56 degrees C, 30 min), or sonication did not alter its stimulatory activity. Polymyxin B, which strongly inhibited the effect of LPS, did not exert any influence on the stimulatory activity of B. henselae. Furthermore, the effect of sonicated B. henselae was not inhibited even by boiling, which was also the case with LPS. Our data suggest that some heat-stable component of B. henselae binds to the endothelial cell surface, inducing ICAM-1 expression. Though the participation of LPS could not be completely ruled out, we suppose that some unidentified heat-stable proteins, lipids, or polysaccharides may be the stimulatory factor(s). The ability of B. henselae to enhance the expression of adhesion molecules on endothelial cells may be an important mechanism in the pathogenesis of B. henselae infection. |
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Authors:
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N Maeno; K Yoshiie; S Matayoshi; T Fujimura; S Mao; M R Wahid; H Oda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of immunology Volume: 55 ISSN: 0300-9475 ISO Abbreviation: Scand. J. Immunol. Publication Date: 2002 Apr |
Date Detail:
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Created Date: 2002-04-22 Completed Date: 2002-05-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: England |
Other Details:
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Languages: eng Pagination: 366-72 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan. maeno@m3.kufm.kagoshima-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bartonella henselae / pathogenicity* Cells, Cultured Endothelium, Vascular / cytology, metabolism* Fimbriae, Bacterial / physiology Hot Temperature Humans Intercellular Adhesion Molecule-1 / biosynthesis* Lipopolysaccharides / toxicity Mice Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 126547-89-5/Intercellular Adhesion Molecule-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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