Document Detail


A heat-inducible cre/loxP gene induction system in medaka.
MedLine Citation:
PMID:  23019184     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We established three lines of transgenic medaka, a heat-shock element (HSE) monitor line (hse-GFP line), heat-inducible driver lines (hse-cre lines), and effector lines (gapdh-loxP[DsRed]-GFP lines). We employed these to comprehensively analyze gene induction at different time points in various tissues. These analyses demonstrate a good response of synthetic HSEs by heat treatment during embryogenesis and the mosaic gene induction by cre/loxP-mediated recombination, thus providing practical informations regarding the feasibility of a heat-inducible cre/loxP-mediated system in medaka. We also activated recombination by local heat-treatment using a metal probe and an infra-red laser. Our results collectively indicate that these lines allow us to perform lineage tracing and mosaic analysis and provide the platform to investigate gene functions at later developmental stage and adult. © 2012 Wiley Periodicals, Inc.
Authors:
Kayo Kobayashi; Yasuhiro Kamei; Masato Kinoshita; Thomas Czerny; Minoru Tanaka
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-28
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  -     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki 444-8787, Japan.
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