| A hard microflow cytometer using groove-generated sheath flow for multiplexed bead and cell assays. | |
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MedLine Citation:
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PMID: 20658281 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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With a view toward developing a rugged microflow cytometer, a sheath flow system was micromachined in hard plastic (polymethylmethacrylate) for analysis of particles and cells using optical detection. Six optical fibers were incorporated into the interrogation region of the chip, in which hydrodynamic focusing narrowed the core stream to ~35 μm × 40 μm. The use of a relatively large channel at the inlet as well as in the interrogation region (375 μm × 125 μm) successfully minimized the risk of clogging. The device could withstand pressures greater than 100 psi without leaking. Assays using both coded microparticles and cells were demonstrated using the microflow cytometer. Multiplexed immunoassays detected nine different bacteria and toxins using a single mixture of coded microspheres. A549 cancer cells processed with locked nucleic acid probes were evaluated using fluorescence in situ hybridization. |
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Authors:
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Abel L Thangawng; Jason S Kim; Joel P Golden; George P Anderson; Kelly L Robertson; Vyechi Low; Frances S Ligler |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-07-25 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 398 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-20 Completed Date: 2011-02-09 Revised Date: 2013-05-29 |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1871-81 Citation Subset: IM |
Affiliation:
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Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Assay
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instrumentation*,
methods Cell Line, Tumor Flow Cytometry / instrumentation*, methods* Humans Immunoassay Microspheres Polymethyl Methacrylate / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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U01 A1075489//PHS HHS; U01 AI075489/AI/NIAID NIH HHS; U01 AI075489-03/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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9011-14-7/Polymethyl Methacrylate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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