| hTERT Gene Immortalized Human Adipose-Derived Stem Cells and its Multiple Differentiations: a Preliminary Investigation. | |
| | |
MedLine Citation:
|
PMID: 23322249 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Human adipose-derived adult stem cells (hADSCs) can express human telomerase reverse transcriptase phenotypes under an appropriate culture condition. Because adipose tissue is abundant and easily accessible, hADSCs offer a promising source of stem cells for tissue engineering application and other cell-based therapies. However, the shortage of cells number and the difficulty to proliferate, known as the "Hayflick limit" in vitro, limit their further clinical application. Here, hADSCs were transfected with human telomerase reverse transcriptase (hTERT) gene by the lentiviral vector to prolong the lifespan of stem cells and even immortalize them. Following to this, the cellular properties and functionalities of the transfected cell lines were assayed. The results demonstrated that hADSCs had been successfully transfected with hTERT gene (hTERT-ADSCs). Then, hTERT-ADSCs were initially selected by G418 and subsequently expanded over 20 passages in vitro. Moreover, the qualitative and quantitative differentiation criteria for 20 passages of hTERT-ADSCs also demonstrated that hTERT-ADSCs could differentiate into osteogenesis, chondrogenesis, and adipogenesis phenotypes in lineage-specific differentiation media. These findings confirmed that this transfection could prolong the lifespan of hADSCs. |
| | |
Authors:
|
L Wang; K Song; X Qu; H Wang; H Zhu; X Xu; M Zhang; Y Tang; X Yang |
Related Documents
:
|
23255099 - Molecular regulation of determination in asymmetrically dividing muscle stem cells. 23395909 - Soluble ligands for the nkg2d receptor are released during hiv-1 infection and impair n... 23337809 - Helios marks strongly autoreactive cd4+ t cells in two major waves of thymic deletion d... 23054339 - Treatment with low doses of polyclonal immunoglobulin improves b cell function during i... 23693139 - Ovarian stem cells-potential roles in infertility treatment and fertility preservation. 23245119 - 285 generation of canine induced pluripotent stem cells from canine fetal fibroblast an... 2496549 - Central nervous system involvement demonstrated by immunological study in prolymphocyti... 9707509 - Direct exposure to gallium arsenide upregulates costimulatory activity of murine macrop... 18216199 - Constitutive egfr signaling in oligodendrocyte progenitors leads to diffuse hyperplasia... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-16 |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Oncology, First Affiliated Hospital of Dalian Medical University, Dalian, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A qualitative and quantitative analysis of the characteristics of gout patient education resources.
Next Document: Advances in RNA Interference Technology and Its Impact on Nutritional Improvement, Disease and Insec...