Document Detail


hRad21 overexpresses and localizes to the ALT-associated promyelocytic leukemia body in ALT cells.
MedLine Citation:
PMID:  20364118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Telomerase-negative immortalized cells maintain their telomeres through a telomerase-independent pathway termed alternative lengthening of telomeres (ALT). The mechanism of ALT is based on homologous recombination (HR). A hallmark of ALT cells is presence of a nuclear structure termed ALT-associated promyelocytic leukemia body (APB). Here, we demonstrated that hRAD21, an important subunit of cohesin complex, was overexpressed in ALT cells. We additionally showed that hRAD21 protein localized to APB in ALT cells. Thus, one role of hRAD21 appeared to involve telomere maintenance in ALT cells. We suggested that hRAD21 facilitated telomere HR in ALT cells by participating in APB formation.
Authors:
Bo Zhao; Zhen Jun Wang; Bing Qiang Yi; Hua Chong Ma; Hui Min Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-26
Journal Detail:
Title:  Cancer biology & therapy     Volume:  9     ISSN:  1555-8576     ISO Abbreviation:  Cancer Biol. Ther.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-09-21     Completed Date:  2011-02-18     Revised Date:  2011-03-30    
Medline Journal Info:
Nlm Unique ID:  101137842     Medline TA:  Cancer Biol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  978-83     Citation Subset:  IM    
Affiliation:
Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Chaoyang District, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle Proteins / genetics,  metabolism
Cell Line
Cell Line, Tumor
Chromosomal Proteins, Non-Histone / genetics,  metabolism
DNA Repair
Gene Expression
Humans
Intranuclear Inclusion Bodies / metabolism*
Multiprotein Complexes / metabolism
Nuclear Proteins / genetics,  metabolism*
Phosphoproteins / genetics,  metabolism*
Recombination, Genetic*
Telomerase / genetics,  metabolism
Telomere / genetics,  metabolism*
Telomeric Repeat Binding Protein 2 / genetics,  metabolism
Transcription Factors / metabolism
Tumor Suppressor Proteins / metabolism
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Multiprotein Complexes; 0/Nuclear Proteins; 0/Phosphoproteins; 0/RAD21 protein, human; 0/Telomeric Repeat Binding Protein 2; 0/Transcription Factors; 0/Tumor Suppressor Proteins; 0/cohesins; 143220-95-5/PML protein, human; EC 2.7.7.49/Telomerase
Comments/Corrections
Comment In:
Cancer Biol Ther. 2010 Jun;9(12):984-5   [PMID:  20431348 ]

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