Document Detail


hOLFML1, a novel secreted glycoprotein, enhances the proliferation of human cancer cell lines in vitro.
MedLine Citation:
PMID:  18708057     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a novel secreted protein, named hOLFML1 (human olfactomedin-like protein 1), with an olfectamine domain in its C-terminus, mainly expressed in the small intestine, liver, lung and heart. Immunohistochemical staining on human small intestine indicated that the protein localizes preferentially in the intestinal villi. Interestingly, ectopic hOLFML1 promoted proliferation of HeLa cells and increased the percentage of cells in S phase. In contrast, knock down of hOLFML1 protein expression by siRNA inhibited cell proliferation and delayed the entry of cells into S phase. Our data also revealed that hOLFML1 is N-glycosylated and its secretion is triggered by serum. Taken together, these findings suggest that hOLFML1 may play a significant role in the regulation of cell proliferation in vitro.
Authors:
Bingbing Wan; Yu-Bo Zhou; Xin Zhang; Hong Zhu; Keke Huo; Ze-Guang Han
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-15
Journal Detail:
Title:  FEBS letters     Volume:  582     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-15     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3185-92     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University and Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai 201203, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
COS Cells
Cell Proliferation*
Cercopithecus aethiops
Conserved Sequence
Extracellular Matrix Proteins / classification,  genetics,  metabolism*
Glycoproteins / classification,  genetics,  metabolism*
Hela Cells
Humans
Intestine, Small / metabolism
Phylogeny
RNA, Small Interfering / genetics
Tissue Distribution
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Glycoproteins; 0/RNA, Small Interfering; 0/olfactomedin

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