| hCG responsiveness of purified Leydig cells from immature and mature rats. | |
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MedLine Citation:
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PMID: 6301963 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Collagenase dispersed cells from immature or mature rat testis were separated into 11 bands on a discontinuous Percoll gradient. Erythrocytes sedimented at the bottom. Only three bands (8, 9 and 10) bind efficiently [125I]hCG. In immature rats, cells in bands 8, 9 and 10 bind hCG with the same affinity, but differ slightly in their total number of sites (average 65,000 +/- 4,000 sites per Leydig cell) and in their cAMP and testosterone production in response to hCG. They present 8% of the total number of dispersed cells. In mature rat testis, populations 8, 9 and 10 differed markedly in their capacity to bind [125I]hCG and in their response to hCG as regards an increase in cAMP and testosterone production. Testosterone production was increased 2- and 17-fold in bands 8 and 10, respectively. In mature rats only 1.2% of the total cells are Leydig cells. In both mature and immature rats, band 10 is made of 100% Leydig cells, as all cells stained positively for 3 beta-hydroxysteroid dehydrogenase. |
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Authors:
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A Lefèvre; J M Saez; C Finaz |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Hormone research Volume: 17 ISSN: 0301-0163 ISO Abbreviation: Horm. Res. Publication Date: 1983 |
Date Detail:
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Created Date: 1983-06-10 Completed Date: 1983-06-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0366126 Medline TA: Horm Res Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 114-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging Animals Chorionic Gonadotropin / pharmacology* Cyclic AMP / biosynthesis Histocytochemistry Leydig Cells / drug effects* Male Rats Rats, Inbred Strains Testosterone / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 58-22-0/Testosterone; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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