| A guide to ions and RNA structure. | |
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MedLine Citation:
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PMID: 14970378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RNA folding into stable tertiary structures is remarkably sensitive to the concentrations and types of cations present; an understanding of the physical basis of ion-RNA interactions is therefore a prerequisite for a quantitative accounting of RNA stability. This article summarizes the energetic factors that must be considered when ions interact with two different RNA environments. "Diffuse ions" accumulate near the RNA because of the RNA electrostatic field and remain largely hydrated. A "chelated" ion directly contacts a specific location on the RNA surface and is held in place by electrostatic forces. Energetic costs of ion chelation include displacement of some of the waters of hydration by the RNA surface and repulsion of diffuse ions. Methods are discussed for computing both the free energy of the set of diffuse ions associated with an RNA and the binding free energies of individual chelated ions. Such calculations quantitatively account for the effects of Mg(2+) on RNA stability where experimental data are available. An important conclusion is that diffuse ions are a major factor in the stabilization of RNA tertiary structures. |
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Authors:
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David E Draper |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S.; Review |
Journal Detail:
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Title: RNA (New York, N.Y.) Volume: 10 ISSN: 1355-8382 ISO Abbreviation: RNA Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-02-18 Completed Date: 2004-03-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 9509184 Medline TA: RNA Country: United States |
Other Details:
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Languages: eng Pagination: 335-43 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA. draper@jhu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Humans Ions / metabolism* Magnesium / metabolism Nucleic Acid Conformation Potassium / metabolism RNA / chemistry*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM58545/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ions; 63231-63-0/RNA; 7439-95-4/Magnesium; 7440-09-7/Potassium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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