| The growth phase-dependent regulation of the pilus locus genes by two-component system TCS08 in Streptococcus pneumoniae. | |
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MedLine Citation:
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PMID: 18983906 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The two-component system TCS08 of Streptococcus pneumoniae contributes to the virulence in vivo and regulates phosphotransferase system (PTS) genes in an avirulent strain. However, its role in pathogenic strains and virulence mechanism are largely unknown. In this study, we constructed TCS08 knockout mutants in a serotype 4 encapsulated pathogenic strain TIGR4, and investigated target genes regulated by TCS08 through transcriptional profile analysis. Compared to TIGR4, expression of the rlrA islet genes (SP0461-SP0468) encoding pneumococcal pili was found to be up-regulated in the rr08 mutant (Deltarr08). Further quantitative real-time PCR (qRT-PCR) analysis revealed that such induction was more significant when the strains were grown to late-logarithmic phase. In phenotype analyses, disruption of both hk08 and rr08 genes (DeltaTCS08) resulted in increased adherence to human lung epithelial cells (A549) at 3 h at late-logarithmic and stationary phases. However, the invasion level of DeltaTCS08 was reduced at different growth phases. Similar phenotype changes, though less significant, were also observed when the assays were performed on human nasopharyngeal epithelial cells (Detroit 562). These data suggest that TCS08 is involved in adhesion and invasion of host epithelial cells, which is likely mediated via regulation of the pilus locus genes in a growth phase-dependent manner. |
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Authors:
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Xin-Ming Song; Wayne Connor; Karsten Hokamp; Lorne A Babiuk; Andrew A Potter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-17 |
Journal Detail:
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Title: Microbial pathogenesis Volume: 46 ISSN: 0882-4010 ISO Abbreviation: Microb. Pathog. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-02 Completed Date: 2009-04-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8606191 Medline TA: Microb Pathog Country: England |
Other Details:
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Languages: eng Pagination: 28-35 Citation Subset: IM |
Affiliation:
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Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, S7N 5E3 Canada. xinming.song@usask.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion Bacterial Proteins / genetics, metabolism* Cell Line Epithelial Cells / cytology, microbiology Fimbriae, Bacterial / genetics, metabolism* Gene Deletion Gene Expression Regulation, Bacterial* Humans Lung / cytology, microbiology Nasopharynx / cytology, microbiology Oligonucleotide Array Sequence Analysis RNA, Bacterial / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* Streptococcus pneumoniae / genetics, growth & development*, metabolism, pathogenicity Trans-Activators / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/RNA, Bacterial; 0/Trans-Activators; 0/rlrA protein, Streptococcus pneumoniae |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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