Document Detail

The gradual onset brain death model: a relevant model to study organ donation and its consequences on the outcome after transplantation.
MedLine Citation:
PMID:  17640464     Owner:  NLM     Status:  MEDLINE    
Organs used for transplantation are usually derived from heart-beating brain dead donors. However, brain death is known to have negative effects on donor organ quality, previously studied using a difficult to control sudden onset experimental model. We have now developed a reproducible gradual onset brain death model in rats without requiring inotropic support. Fisher inbred rats weighing 260-300 g were used. Brain death was induced by a gradual inflation of a subdurally placed balloon catheter. During induction and the period following brain death, the animals were mechanically ventilated and blood pressure was continuously monitored. The blood pressure registration showed a characteristic pattern during brain death induction, in which a decrease in blood pressure, a hypotensive period in which the Cushing response occurred, and a sharp peak were consistent findings. After brain death was induced, blood pressure was maintained at normotensive levels up to 4 h. After the experiments, neuropathological evaluation of the brain located haemorrhagic cerebral parenchyma, and immunocytochemistry of liver tissue revealed a significant influx of polymorph nuclear cells, as was previously observed as well. This improved model allows the study of brain death on donor organ quality without the use of inotropic support.
Joe L P Kolkert; Nils A 't Hart; Antony van Dijk; Petra J Ottens; Rutger J Ploeg; Henri G D Leuvenink
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Laboratory animals     Volume:  41     ISSN:  0023-6772     ISO Abbreviation:  Lab. Anim.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-20     Completed Date:  2007-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0112725     Medline TA:  Lab Anim     Country:  England    
Other Details:
Languages:  eng     Pagination:  363-71     Citation Subset:  IM    
Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
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MeSH Terms
Biological Markers / metabolism
Blood Pressure
Brain / pathology
Brain Death / pathology,  physiopathology*
Disease Models, Animal*
Immunoenzyme Techniques
Liver / metabolism,  pathology
Organ Preservation*
Organ Transplantation / methods*
Rats, Inbred F344
Respiration, Artificial
Tissue Donors*
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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