| The gradual onset brain death model: a relevant model to study organ donation and its consequences on the outcome after transplantation. | |
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MedLine Citation:
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PMID: 17640464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Organs used for transplantation are usually derived from heart-beating brain dead donors. However, brain death is known to have negative effects on donor organ quality, previously studied using a difficult to control sudden onset experimental model. We have now developed a reproducible gradual onset brain death model in rats without requiring inotropic support. Fisher inbred rats weighing 260-300 g were used. Brain death was induced by a gradual inflation of a subdurally placed balloon catheter. During induction and the period following brain death, the animals were mechanically ventilated and blood pressure was continuously monitored. The blood pressure registration showed a characteristic pattern during brain death induction, in which a decrease in blood pressure, a hypotensive period in which the Cushing response occurred, and a sharp peak were consistent findings. After brain death was induced, blood pressure was maintained at normotensive levels up to 4 h. After the experiments, neuropathological evaluation of the brain located haemorrhagic cerebral parenchyma, and immunocytochemistry of liver tissue revealed a significant influx of polymorph nuclear cells, as was previously observed as well. This improved model allows the study of brain death on donor organ quality without the use of inotropic support. |
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Authors:
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Joe L P Kolkert; Nils A 't Hart; Antony van Dijk; Petra J Ottens; Rutger J Ploeg; Henri G D Leuvenink |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Laboratory animals Volume: 41 ISSN: 0023-6772 ISO Abbreviation: Lab. Anim. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-20 Completed Date: 2007-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0112725 Medline TA: Lab Anim Country: England |
Other Details:
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Languages: eng Pagination: 363-71 Citation Subset: IM |
Affiliation:
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Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Blood Pressure Brain / pathology Brain Death / pathology, physiopathology* Disease Models, Animal* Immunoenzyme Techniques Liver / metabolism, pathology Male Organ Preservation* Organ Transplantation / methods* Rats Rats, Inbred F344 Respiration, Artificial Tissue Donors* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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