Document Detail


(-)-Gossypol reduces invasiveness in metastatic prostate cancer cells.
MedLine Citation:
PMID:  19528479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Acquisition of metastatic ability by prostatic cancer cells is the most lethal aspect of prostatic cancer progression. (-)-Gossypol, a polyphenolic compound present in cottonseeds, possesses anti-proliferative and proapoptotic effects in various cancer cells. MATERIALS AND METHODS: In this study, the differences between MAT-LyLu, rat prostate cancer cells, with a novel isolated subline from metastasized tumors in the lungs of MAT-LyLu-bearing Copenhagen rats (MLL cells) were compared with respect to cell growth and invasion. The effects of (-)-gossypol on cell viability, colony formation, invasive ability and cell migration in MAT-LyLu and MLL cells were also evaluated. RESULTS: Results showed that MLL cells displayed higher growth ability, colony formation and aggressive penetration than those of MAT-LyLu cells. MLL cells possess lower protein expression of Bcl-xL and nm23-H1 than those of MAT-LyLu cells, implying differences in invasive ability. Moreover, (-)-gossypol treatment induced a dose-dependent inhibition of invasive activity and cell viability and reduced Bcl-2 and Bcl-xL proteins but induced nm23-H1 protein in both cell lines. CONCLUSION: These findings illustrated that (-)-gossypol reduced in vitro invasion of both the parental MAT-LyLu cells and the isolated MLL cells, suggesting that (-)-gossypol might serve as a chemotherapeutic and/or chemopreventive agent.
Authors:
Yi-Wen Huang; Li-Shu Wang; Michael K Dowd; Peter J Wan; Young C Lin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  29     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-16     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  2179-88     Citation Subset:  IM    
Affiliation:
Laboratory of Reproductive and Molecular Endocrinology, College of Veterinary Medicine, The Ohio State University, Columbus, OH, U.S.A.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Blotting, Western
Cell Movement / drug effects
Cell Proliferation / drug effects
Contraceptive Agents, Male / therapeutic use*
Gossypol / therapeutic use*
Lung Neoplasms / drug therapy*,  metabolism,  secondary
Male
Neoplasm Invasiveness
Prostatic Neoplasms / drug therapy*,  metabolism,  pathology*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Rats
bcl-X Protein / metabolism
Grant Support
ID/Acronym/Agency:
CA94718/CA/NCI NIH HHS; CA95915/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Contraceptive Agents, Male; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-X Protein; 303-45-7/Gossypol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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