Document Detail

The glycosylphosphatidylinositol-anchored phosphatase from Spirodela oligorrhiza is a purple acid phosphatase.
MedLine Citation:
PMID:  9808746     Owner:  NLM     Status:  MEDLINE    
We recently presented clear evidence that the major low-phosphate-inducible phosphatase of the duckweed Spirodela oligorrhiza is a glycosylphosphatidylinositol (GPI)-anchored protein, and, to our knowledge, is the first described from higher plants (N. Morita, H. Nakazato, H. Okuyama, Y. Kim, G.A. Thompson, Jr. [1996] Biochim Biophys Acta 1290: 53-62). In this report the purified 57-kD phosphatase is shown to be a purple metalloenzyme containing Fe and Mn atoms and having an absorption maximum at 556 nm. The phosphatase activity was only slightly inhibited by tartrate, as expected for a purple acid phosphatase (PAP). Furthermore, the protein cross-reacted with an anti-Arabidopsis PAP antibody on immunoblots. The N-terminal amino acid sequence of the phosphatase was very similar to those of Arabidopsis, red kidney bean (Phaseolus vulgaris), and soybean (Glycine max) PAP. Extracts of S. oligorrhiza plants incubated with the GPI-specific precursor [3H]ethanolamine were treated with antibodies raised against the purified S. oligorrhiza phosphatase. Radioactivity from the resulting immunoprecipitates was specifically associated with a 57-kD band on sodium dodecyl sulfate-polyacrylamide gels. These results, together with previous findings, strongly indicate that the GPI-anchored phosphatase of S. oligorrhiza is a PAP.
H Nakazato; T Okamoto; M Nishikoori; K Washio; N Morita; K Haraguchi; G A Thompson; H Okuyama
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Plant physiology     Volume:  118     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-01-14     Completed Date:  1999-01-14     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1015-20     Citation Subset:  IM    
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo 060-0810, Japan.
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MeSH Terms
Acid Phosphatase / antagonists & inhibitors,  chemistry,  metabolism*
Amino Acid Sequence
Blotting, Western
Enzyme Inhibitors / pharmacology
Glycoproteins / antagonists & inhibitors,  chemistry,  metabolism*
Glycosylphosphatidylinositols / metabolism*
Metals / analysis
Molecular Sequence Data
Plants / enzymology*
Sequence Homology, Amino Acid
Spectrum Analysis
Tartrates / pharmacology
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Glycoproteins; 0/Glycosylphosphatidylinositols; 0/Metals; 0/Tartrates; 10028-17-8/Tritium; 141-43-5/Ethanolamine; EC 3.1.3.-/purple acid phosphatase; EC Phosphatase

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