| A glycoprotein extracted from golden oyster mushroom Pleurotus citrinopileatus exhibiting growth inhibitory effect against U937 leukemia cells. | |
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MedLine Citation:
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PMID: 19606865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. Golden oyster mushroom, Pleurotus citrinopileatus , is a common mushroom in oriental countries for human consumption. We isolated a functional protein (PCP-3A) from the fresh fruiting body of this mushroom. The isolation procedure included ammonium sulfate fractionation, DEAE-Sepharose CL-6B ion exchange chromatography, and Sephacryl S-300 gel filtration. Electrophoresis demonstrated that PCP-3A is a glycoprotein composed of 10 subunits, each approximately 45.0 kDa in size. In vitro cell study showed that PCP-3A at a concentration about 12.5 microg/mL inhibits the proliferation of human tumor cell line U937, in a time- dependent manner (24, 48, and 72 h). It failed to agglutinate rabbit and human erythrocytes, excluding its possibility from being a lectin. Flow cytometry revealed that it is capable of inhibiting the growth of U937 cells by way of S phase arrest and apoptotic induction. We suggest that PCP-3A is worth further investigating for antitumor use. |
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Authors:
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Jian-Nan Chen; Yuh-Tai Wang; James Swi-Bea Wu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 57 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-09-02 Completed Date: 2009-11-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6706-11 Citation Subset: IM |
Affiliation:
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Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Cell Proliferation / drug effects* Erythrocytes / drug effects, immunology Fruiting Bodies, Fungal / chemistry Fungal Proteins / chemistry, isolation & purification, pharmacology* Glycoproteins / chemistry, isolation & purification, pharmacology* Hemagglutination / drug effects Humans Leukemia / drug therapy, immunology, physiopathology* Pleurotus / chemistry* Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Glycoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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