Document Detail


A glycoprotein extracted from golden oyster mushroom Pleurotus citrinopileatus exhibiting growth inhibitory effect against U937 leukemia cells.
MedLine Citation:
PMID:  19606865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. Golden oyster mushroom, Pleurotus citrinopileatus , is a common mushroom in oriental countries for human consumption. We isolated a functional protein (PCP-3A) from the fresh fruiting body of this mushroom. The isolation procedure included ammonium sulfate fractionation, DEAE-Sepharose CL-6B ion exchange chromatography, and Sephacryl S-300 gel filtration. Electrophoresis demonstrated that PCP-3A is a glycoprotein composed of 10 subunits, each approximately 45.0 kDa in size. In vitro cell study showed that PCP-3A at a concentration about 12.5 microg/mL inhibits the proliferation of human tumor cell line U937, in a time- dependent manner (24, 48, and 72 h). It failed to agglutinate rabbit and human erythrocytes, excluding its possibility from being a lectin. Flow cytometry revealed that it is capable of inhibiting the growth of U937 cells by way of S phase arrest and apoptotic induction. We suggest that PCP-3A is worth further investigating for antitumor use.
Authors:
Jian-Nan Chen; Yuh-Tai Wang; James Swi-Bea Wu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6706-11     Citation Subset:  IM    
Affiliation:
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Cell Proliferation / drug effects*
Erythrocytes / drug effects,  immunology
Fruiting Bodies, Fungal / chemistry
Fungal Proteins / chemistry,  isolation & purification,  pharmacology*
Glycoproteins / chemistry,  isolation & purification,  pharmacology*
Hemagglutination / drug effects
Humans
Leukemia / drug therapy,  immunology,  physiopathology*
Pleurotus / chemistry*
Rabbits
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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