| The glycoconjugate sugar residues of the sessile and motile cells in the thymus of normal and cyclosporin-A-treated rats: lectin histochemistry. | |
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MedLine Citation:
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PMID: 11820218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is well known that cell surface glycoconjugates play a determinant role in cellular recognition, cell-to-cell adhesion and serve as receptor molecules. T-lymphocytes are in strict contact with the thymic epithelial cells, which control their process of maturation and proliferation. On the other hand the normal maturation of the epithelial cells is believed to be induced by T-lymphocytes. For these reasons we have studied the glycoconjugates saccharidic moieties of the sessile and motile cells in the thymus of normal male albino Wistar rats and their changes following cyclosporin-A treatment, using a battery of seven HRP-lectins. Cytochemical controls were performed for specificity of lectin-sugar reaction. Some sections were pre-treated with neuraminidase prior to staining with HRP-lectins. Our results have demonstrated, in the control rats, a large amount and a variety of terminal and subterminal oligosaccharides within and/or on the epithelial thymic cells and in macrophages. After cyclosporin-A treatment, among the thymic epithelial cells, the subcapsular, paraseptal and perivascular cells showed the loss of some sugar residues, which characterized the same cells in the intact thymus. Some hypotheses are reported on the role played by the glycoconjugate sugar residues in control and cyclosporin-A treated rats. |
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Authors:
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G Gheri; S Gheri Bryk; R Riccardi; E Sgambati; M B Cirri Borghi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histology and histopathology Volume: 17 ISSN: 0213-3911 ISO Abbreviation: Histol. Histopathol. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2002-01-29 Completed Date: 2002-07-16 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
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Languages: eng Pagination: 9-19 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Histology and Forensic Medicine, Policlinico di Careggi, Florence, Italy. gheri@unifi.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atrophy Carbohydrate Metabolism* Cell Movement / drug effects, physiology Cyclosporine / pharmacology* Glycoconjugates / metabolism* Histocytochemistry Horseradish Peroxidase Immunosuppressive Agents / pharmacology* Lectins Male Neuraminidase / chemistry Rats Rats, Wistar Thymus Gland / cytology*, drug effects*, metabolism Tissue Fixation |
| Chemical | |
Reg. No./Substance:
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0/Glycoconjugates; 0/Immunosuppressive Agents; 0/Lectins; 59865-13-3/Cyclosporine; EC 1.11.1.-/Horseradish Peroxidase; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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