Document Detail


The glycerophosphoryl diester phosphodiesterase-like proteins SHV3 and its homologs play important roles in cell wall organization.
MedLine Citation:
PMID:  18718934     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Despite the importance of extracellular events in cell wall organization and biogenesis, the mechanisms and related factors are largely unknown. We isolated an allele of the shaven3 (shv3) mutant of Arabidopsis thaliana, which exhibits ruptured root hair cells during tip growth. SHV3 encodes a novel protein with two tandemly repeated glycerophosphoryl diester phosphodiesterase-like domains and a glycosylphosphatidylinositol anchor, and several of its paralogs are found in Arabidopsis. Here, we report the detailed characterization of mutants of SHV3 and one of its paralogs, SVL1. The shv3 and svl1 double mutant exhibited additional defects, including swollen guard cells, aberrant expansion of the hypocotyl epidermis and ectopic lignin deposits, suggesting decreased rigidity of the cell wall. Fourier-transform infrared spectroscopy and measurement of the cell wall components indicated an altered cellulose content and pectin modification with cross-linking in the double mutant. Furthermore, we found that the ruptured root hair phenotype of shv3 was suppressed by increasing the amount of borate, which is supposed to be involved in pectic polysaccharide cross-linking, in the medium. These findings indicate that SHV3 and its paralogs are novel important factors involved in primary cell wall organization.
Authors:
Shimpei Hayashi; Tadashi Ishii; Toshiro Matsunaga; Rumi Tominaga; Takashi Kuromori; Takuji Wada; Kazuo Shinozaki; Takashi Hirayama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-20
Journal Detail:
Title:  Plant & cell physiology     Volume:  49     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-14     Completed Date:  2008-11-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1522-35     Citation Subset:  IM    
Affiliation:
International Graduate School of Arts and Sciences, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama, 230-0045 Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Arabidopsis / cytology,  enzymology*,  genetics
Arabidopsis Proteins / genetics,  metabolism*
Cell Wall / genetics,  metabolism*,  ultrastructure
Cellulose / metabolism
DNA, Bacterial / genetics
Genes, Plant
Hypocotyl / cytology,  enzymology,  genetics
Microscopy, Electron, Scanning
Molecular Sequence Data
Pectins / metabolism
Phenotype
Phosphoric Diester Hydrolases / genetics,  metabolism*
Plant Epidermis / ultrastructure
Plant Roots / cytology,  enzymology,  genetics
Plants, Genetically Modified / cytology,  enzymology,  genetics
Point Mutation
RNA, Plant / genetics
Spectroscopy, Fourier Transform Infrared
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA, Bacterial; 0/Pectins; 0/RNA, Plant; 0/T-DNA; 9000-69-5/pectin; 9004-34-6/Cellulose; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.1.4.46/glycerophosphodiester phosphodiesterase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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