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The Sda/GM2-glycan is a carbohydrate marker of porcine primordial germ cells and of a subpopulation of spermatogonia in cattle, pigs, horses and llama.
MedLine Citation:
PMID:  21896636     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Spermatogonia are a potential source of adult pluripotent stem cells and can be used for testis germ cell transplantation. Markers for the isolation of these cells are of great importance for biomedical applications. Primordial germ cells and prepubertal spermatogonia in many species can be identified by their binding of Dolichos biflorus agglutinin (DBA). This lectin binds to two different types of glycans, which are α-linked N-acetylgalactosamine(GalNac) and β-linked GalNac, if this is part of the Sda- or GM2-glycotopes. We used the monoclonal antibody CT1, which is specific for the trisaccharids motif NeuAcα2-3(GalNAcβ1-4)Galβ1-, which is common to both Sda and GM2 glycotopes, to further define the glycosylation of DBA binding germ cells. In porcine embryos CT1 bound to migratory germ cells and gonocytes. CT1/DBA doublestaining showed that the mesonephros was CT1-negative, but contained DBA-positive cells. Gonocytes in the female gonad became CT1-negative, while male gonocytes remained CT1 positive. In immunohistological doublestainings of cattle, pig, horse, and llama testis DBA- and CT1-staining was generally colocalised in a subpopulation of spermatogonia. These spermatogonia were mainly single, sometimes paired or formed chains of up to four cells. Our data show that the Sda/GM2-glycotope is present in developing germ cells and spermatogonia in several species. Due to the narrower specificity of the CT1antibody, compared to DBA, the former is likely to be a useful tool for labelling and isolation of these cells.
Authors:
Karl Klisch; David Alejandro Contreras; Xiaoni Sun; Ralph Brehm; Martin Bergmann; Ramiro Alberio
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-6
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  -     ISSN:  1741-7899     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
K Klisch, School of Veterinary Medicine and Science, University of Nottingham, Loughborough, United Kingdom.
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