Document Detail


The glutamic acid decarboxylase system of the new species Brucella microti contributes to its acid resistance and to oral infection of mice.
MedLine Citation:
PMID:  22930809     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background. Genome analysis indicated that the new species Brucella microti possesses a potentially functional glutamate decarboxylase (GAD) system involved in extreme acid resistance in several food-borne bacteria. The contribution of this system in adaptation of B. microti to acid environment, including the intracellular vacuole and stomach, was investigated.Results. B. microti was GAD-positive and able to export its product, γ-aminobutyrate (GABA), to the extracellular medium. The resistance of B. microti to pH 2.5 was glutamate-dependent. Mutants affected in the GAD system lost this resistance, demonstrating its direct involvement in survival under these conditions. The reciprocal heterologous complementation of mutants with the GAD systems of E. coli or B. microti confirmed conserved functions in both bacterial species. A gad-mutant was not attenuated during macrophage infection, where Brucella resides in an acidified vacuole at pH 4-4.5 during early phase, but GAD contributed to survival of B. microti in a murine model following oral infection.Conclusion. This work provides first evidence that the GAD system might play an essential role in the resistance of an environment-borne, pathogenic Brucella species to extreme acid shock and during passage of the host stomach following oral infection.
Authors:
Alessandra Occhialini; Maria Pilar Jiménez de Bagüés; Bashir Saadeh; Daniela Bastianelli; Nabil Hanna; Daniela De Biase; Stephan Köhler
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-28
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  -     ISSN:  1537-6613     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Université Montpellier I, Centre d'études d'agents Pathogènes et Biotechnologie pour la Santé (CPBS).
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