Document Detail

The glutamate-dependent acid resistance system of Escherichia coli and Shigella flexneri is inhibited in vitro by L-trans-pyrrolidine-2,4-dicarboxylic acid.
MedLine Citation:
PMID:  12855178     Owner:  NLM     Status:  MEDLINE    
Strains of Escherichia coli K-12, O157:H7, and Shigella flexneri grown to stationary phase in complex unbuffered media can survive for several hours at pH 2.5. This stationary-phase acid resistance phenotype is dependent upon the alternate sigma factor sigmas and the supplementation of either glutamate or glutamine in the acidified media used for acid challenge. Acid resistance under these defined conditions can be inhibited by the glutamate analog L-trans-pyrrolidine-2,4-dicarboxylic acid which blocks uptake of glutamate/glutamine by selective inhibition. The gadC gene, encoding an inner membrane antiporter essential for the expression of acid resistance, could not be detected in other family members of the Enterobacteriacae.
Scott R Waterman; P L C Small
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  224     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-11     Completed Date:  2003-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  119-25     Citation Subset:  IM    
Division of Human Immunology, Hanson Institute, IMVS, Adelaide, SA 5000, Australia.
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MeSH Terms
Acids / metabolism
Bacterial Proteins*
Culture Media
DNA, Bacterial / analysis
Dicarboxylic Acids / pharmacology*
Escherichia coli / drug effects*,  genetics,  metabolism*
Escherichia coli Proteins*
Glutamic Acid / analogs & derivatives,  metabolism
Glutamine / metabolism
Membrane Proteins / genetics,  metabolism
Neurotransmitter Uptake Inhibitors / pharmacology*
Pyrrolidines / pharmacology*
Shigella flexneri / drug effects*,  genetics,  metabolism*
Sigma Factor / metabolism
gamma-Aminobutyric Acid / metabolism
Reg. No./Substance:
0/Acids; 0/Bacterial Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/Dicarboxylic Acids; 0/Escherichia coli Proteins; 0/GadC protein, bacteria; 0/Membrane Proteins; 0/Neurotransmitter Uptake Inhibitors; 0/Pyrrolidines; 0/Sigma Factor; 0/xasA protein, E coli; 56-12-2/gamma-Aminobutyric Acid; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 99319-03-6/pyrrolidine-2,4-dicarboxylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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