| The glutamate-dependent acid resistance system of Escherichia coli and Shigella flexneri is inhibited in vitro by L-trans-pyrrolidine-2,4-dicarboxylic acid. | |
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MedLine Citation:
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PMID: 12855178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Strains of Escherichia coli K-12, O157:H7, and Shigella flexneri grown to stationary phase in complex unbuffered media can survive for several hours at pH 2.5. This stationary-phase acid resistance phenotype is dependent upon the alternate sigma factor sigmas and the supplementation of either glutamate or glutamine in the acidified media used for acid challenge. Acid resistance under these defined conditions can be inhibited by the glutamate analog L-trans-pyrrolidine-2,4-dicarboxylic acid which blocks uptake of glutamate/glutamine by selective inhibition. The gadC gene, encoding an inner membrane antiporter essential for the expression of acid resistance, could not be detected in other family members of the Enterobacteriacae. |
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Authors:
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Scott R Waterman; P L C Small |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: FEMS microbiology letters Volume: 224 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-07-11 Completed Date: 2003-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 119-25 Citation Subset: IM |
Affiliation:
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Division of Human Immunology, Hanson Institute, IMVS, Adelaide, SA 5000, Australia. scott.waterman@imvs.sa.gov.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acids
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metabolism Bacterial Proteins* Culture Media DNA, Bacterial / analysis Dicarboxylic Acids / pharmacology* Escherichia coli / drug effects*, genetics, metabolism* Escherichia coli Proteins* Glutamic Acid / analogs & derivatives, metabolism Glutamine / metabolism Membrane Proteins / genetics, metabolism Neurotransmitter Uptake Inhibitors / pharmacology* Phenotype Plasmids Pyrrolidines / pharmacology* Shigella flexneri / drug effects*, genetics, metabolism* Sigma Factor / metabolism gamma-Aminobutyric Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Bacterial Proteins; 0/Culture Media; 0/DNA, Bacterial; 0/Dicarboxylic Acids; 0/Escherichia coli Proteins; 0/GadC protein, bacteria; 0/Membrane Proteins; 0/Neurotransmitter Uptake Inhibitors; 0/Pyrrolidines; 0/Sigma Factor; 0/xasA protein, E coli; 56-12-2/gamma-Aminobutyric Acid; 56-85-9/Glutamine; 56-86-0/Glutamic Acid; 99319-03-6/pyrrolidine-2,4-dicarboxylic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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