Document Detail


The glial cell undergoes apoptosis in the microchaete lineage of Drosophila.
MedLine Citation:
PMID:  12441297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim, showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently of gcm and prospero expression. We suggest that apoptosis is triggered by a cell autonomous mechanism.
Authors:
Pierre Fichelson; Michel Gho
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  130     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-11-20     Completed Date:  2003-02-04     Revised Date:  2008-10-16    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  123-33     Citation Subset:  IM    
Affiliation:
UMR 7622, CNRS-Université Paris VI, 9, Quai St Bernard, 75252 Paris Cedex 05, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Animals, Newborn
Apoptosis / physiology*
Axons / physiology
Cell Lineage
Cell Survival
Cysteine Proteinase Inhibitors / genetics
DNA-Binding Proteins
Drosophila / genetics,  growth & development*
Drosophila Proteins / genetics,  metabolism
Epithelial Cells
Epithelium / growth & development
Gene Expression Regulation, Developmental
Inhibitor of Apoptosis Proteins
Microscopy, Confocal / methods
Nerve Tissue Proteins / genetics,  metabolism
Neuroglia / cytology*
Neuropeptides / genetics,  metabolism
Nuclear Proteins / genetics,  metabolism
Phagocytosis / physiology
Pupa
Sense Organs / cytology*,  growth & development*
Trans-Activators / genetics,  metabolism
Transcription Factors*
Viral Proteins / genetics
Chemical
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/DNA-Binding Proteins; 0/Drosophila Proteins; 0/GCM protein, Drosophila; 0/Inhibitor of Apoptosis Proteins; 0/Nerve Tissue Proteins; 0/Neuropeptides; 0/Nuclear Proteins; 0/Trans-Activators; 0/Transcription Factors; 0/Viral Proteins; 0/inhibitor of apoptosis, Nucleopolyhedrovirus; 0/pros protein, Drosophila; 0/reaper protein, Drosophila; 0/wrinkled protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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