Document Detail


A glass capillary microelectrode based on capillarity and its application to the detection of L-glutamate release from mouse brain slices.
MedLine Citation:
PMID:  12558024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new glass capillary microelectrode for L-glutamate is described using pulled glass capillaries (tip size, approximately 12.5 microm) with a very small volume (approximately 2 microl) of inner solution containing glutamate oxidase (GluOx) and ascorbate oxidase. The operation of the electrode is based on capillary action that samples L-glutamate into the inner solution. The enzyme reaction by GluOx generates hydrogen peroxide that is detected at an Os-gel-HRP polymer modified Pt electrode in a three-electrode configuration. The amperometric response behavior of the electrode was characterized in terms of the capillarity, response time, sensitivity and selectivity for measurements of L-glutamate. The currents at 0 V vs. Ag/AgCl increased linearly with the L-glutamate concentration from 10 to 150 microM for in vitro and in situ calibrations. The response was highly selective to L-glutamate over ascorbate, dopamine, serotonin and other amino acids. The detection of L-glutamate in the extracellular fluids of different regions of mouse hippocampal slices under stimulation of KCl was demonstrated.
Authors:
Kumiko Nakajima; Takashi Yamagiwa; Ayumi Hirano; Masao Sugawara
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  19     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-31     Completed Date:  2003-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  55-60     Citation Subset:  IM    
Affiliation:
Department of Chemistry, College of Humanities and Sciences, Nihon University, Sakurajousui, Setagaya, Tokyo 156-8550, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism*
Calibration
Glass*
Glutamic Acid / metabolism*
Male
Mice
Microelectrodes*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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